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CONSTRUCTION OF FULL LENGTH HEPATITIS A VIRUS CDNA FOR TRANSFECTION

CONSTRUCTION OF FULL LENGTH HEPATITIS A VIRUS CDNA FOR TRANSFECTION
用于转染的全长甲型肝炎病毒 CDNA 的构建
批准号：
4688549
负责人：
J I COHEN
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/4688549
关键词：
Hepatovirus complementary DNA genetic manipulation genetic mapping genome molecular cloning plasmids simian virus 40 tissue /cell culture virus genetics

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中文摘要
翻译
甲型肝炎病毒(HAV)的基因已克隆到pBR322中。六个cDNA 一起跨越甲型肝炎病毒整个基因组的克隆已经被分离出来。 这些克隆已经连接在一起，形成了一个单独的克隆 被认为代表了PBR 322中全长甲型肝炎病毒的cDNA类似物。在……里面 将甲型肝炎病毒基因插入到SV40载体中。转染腺病毒载体 组织培养细胞(体外)和绒猴(体内)都有这些 质粒不能产生甲型肝炎病毒。甲型肝炎病毒基因的精细结构作图 随后表示，大约有40个碱基对在 结扎过程。传染性甲型肝炎病毒全长cDNA克隆的构建 正在进行中。
英文摘要
cDNA from hepatitis A virus (HAV) has been cloned into pBR 322. Six cDNA clones which together span the entire genome of HAV have been isolated. These clones have been ligated together to form a single clone which was thought to represent a cDNA analog of full-length HAV in pBR 322. In addition, the HAV cDNA was inserted into an SV40 vector. Transfection of both tissue culture cells (in vitro) and marmosets (in vivo) with these plasmids failed to generate HAV. Fine structure mapping of the HAV cDNA subsequently indicated that about 40 base pairs had been deleted during the ligation process. Construction of a full-length infectious HAV cDNA clone is in progress.
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会议论文
MOLECULAR GENETICS OF VARICELLA ZOSTER VIRUS
CELLULAR HOMOLOGS OF HERPESVIRUS GENES
MOLECULAR BIOLOGY OF VARICELLA ZOSTER VIRUS INFECTIONS
MOLECULAR BIOLOGY OF TRANSFORMATION BY EPSTEIN-BARR VIRUS
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