HISTAMINE RELEASE FROM BEIGE MOUSE MAST CELLS

米色小鼠是切迪亚克-东综合征的动物模型 天哪。这种动物肥大细胞的大颗粒使 详细分析它们对质膜的胞吐作用。我们有 开发了一种能够同时进行生理和心理测试的仪器阵列 活细胞的解剖实时测量与控制 内部微米，首先用它来测量分泌物的电容 和米色小鼠的吞噬腹膜细胞。我们已经设计了一种 肥大细胞的纯化方法。巨噬细胞表现出更大的电容 尽管巨噬细胞是一个较小的细胞，但与肥大细胞相比。这 悖论提示巨噬细胞高度的膜折叠，一个 发现与其在吞噬细胞中的作用一致。大容量 在肥大细胞融合的瞬间可以看到变化，与 计算出的分泌颗粒的面积。个体肿胀 颗粒也可见。预计同时测量的 肿胀和融合将证明或反驳胞吐作用的渗透学说。

The beige mouse is an animal model for the Chediak-Higashi syndrome in man. The large granules of the mast cell of that animal make possible detailed analysis of their exocytosis to the plasma membrane. We have developed an instrumental array capable of simultaneous physiologic and anatomic real-time measurements of living cells with control of the internal millieu, first using it to measure the capacitance of secretory and phagocytic peritoneal cells from the beige mouse. We have devised a method for purification of mast cells. Macrophages show larger capacitance than the mast cell, although the macrophage is a smaller cell. This paradox suggests a high degree of membrane folding in the macrophage, a finding consistent with its role in phagocytosis. Large capacitance changes are seen at the instant of fusion in mast cells, consistent with the calculated area of the secretory granule. Swelling of individual granules is also seen. It is expected that simultaneous measurement of swelling and fusion will prove or disprove the osmotic theory of exocytosis.