CHARACTERIZATION OF OXYGENATED FATTY ACID METABOLITES BY CAPILLARY GC/MS

该项目的主要目标是应用最先进的天然气 色谱/质谱法分析痕量 脂肪酸代谢物的水平，以努力阐明分子 涉及各种神经病理状态的改变。 这些化合物 通常是以极低水平存在或 这种复杂的混合物，其他技术往往不能提供可靠的 结果 在这项研究的第一阶段，重点是 用于表征人体内的甘草素水平 脑脊液（CSF）。 初步研究表明，脑脊液中的前列腺素水平 从神经功能障碍患者中提取的样本， 正常志愿者的。 这些水平往往接近或低于 报告的CSF检测限为10-100 pg/mL。 中描述的结果 本研究总结了用于证明显著 用于CSF样本分析的较低检测水平 来自不同的患者群体。 特别重要的是 首次将这些方法应用于CSF样本 从戒酒患者身上提取的样本

The principal objective of this project is to apply state-of-the-art gas chromatographic/mass spectrometric methodologies to the analysis of trace levels of fatty acid metabolites in an effort to elucidate the molecular alterations involved in various neuropathological states. These compounds are generally potent agents that are present in extremely low levels or in such complex mixtures that other techniques often cannot provide reliable results. During the first phase of this research, emphasis has been placed on characterizing the levels of prostaglandins in human cerebrospinal fluid (CSF). Preliminary investigations have suggested that prostaglandin levels in CSF drawn from patients with neurological impairment are markedly altered from those of normal volunteers. The levels are often near or below the reported detection limits of 10-100 pg/mL of CSF. The results described in this study summarize the methodologies used to demonstrate significantly lower levels of detection for application to the analysis of CSF samples from a variety of patient groups. Of particular importance is the application of these methodologies for the first time to samples of CSF drawn from alcoholic patients during withdrawal.