IG GENETICS--ONTOGENY AND DIFFERENTIATION OF CELLS OF THE RABBIT IMMUNE SYSTEM

尽管携带K2同种型轻链的IG分子很少， 是由我们的实验室兔子产生的，我们证明了 通过S1保护分析K2基因， 用K2特异性合成寡核苷酸探针的北方分析。 类似地，虽然突变的Basilea兔产生很少或没有Ig， 在J1-b 9型轻链中，我们检测到低水平的b 9 RNA。 我们 我们正在调查这是否是异常拼接的信息，因为我们已经 发现Basilea兔中的K1-b 9基因在 J-C内含子的剪接受体位点。 使用类似的方法，我们没有 然而，检测到的mRNA或基因组DNA序列对应于b5 已在血清学上观察到由b 9/b 9细胞产生的同种异型 用b5抗b 9和LPS体外培养。 因此， 对于潜在同种异型的血清学观察，必须调用。 我们有 还显示，来自第一和第三组的合成寡核苷酸 重链可变区的框架区（FR 1和FR 3） 特异性区分a1和a2兔产生的mRNA。 的 然而，原型FR 1和FR 3序列可能不完全相关， 血清学可检测的VHa决定簇。 一群兔子 Basilea突变体所来源的亲本染色体已经被 开发并显示具有限制性片段长度多态性 K2基因的限制性片段长度多态性（RFLP）也发现在Basilea兔。 这使得链接 K1表型，K2 RFLP和一个新发现的RFLP的研究， 兔T细胞受体链恒定区基因。 了分析和 预测κ轻链同种异型决定簇的位置。 预测的决定因素是外部的，位于或附近的循环，并下降， 两个潜在相互作用区域的集群， 可能出现重叠的表位组。 抗K1抗体的相互作用 在IgG抗半抗原抗体上具有这样的表位的抗体被消除 半抗原介导的抗体与半抗原偶联细胞的解离。

Although few, if any, Ig molecules bearing light chains of the K2 isotype are produced by our laboratory rabbits, we demonstrated transcription of the K2 gene by S1 protection analyses and light-chain sized mRNA by northern analysis with a K2-specific synthetic oligonucleotide probe. Similarly, although mutant Basilea rabbits produce little or no Igs with light chains of the J1-b9 type, we have detected low levels of b9 RNA. We are investigating whether this is aberrantly spliced message since we have found that the K1-b9 gene in Basilea rabbits has a point mutation in the splice acceptor site of the J-C intron. With similar methods, we have not however, detected mRNA or genomic DNA sequences corresponding to the b5 allotype that has been observed serologically to be produced by b9/b9 cells cultured in vitro with b5 anti-b9 and LPS. Thus alternative explanations for serological observations of latent allotypes must be invoked. We have also shown that synthetic oligonucleotides from the first and third framework regions (FR1 and FR3) of variable regions of heavy chains specifically distinguish mRNAs produced by a1 and a2 rabbits. The prototype FR1 and FR3 sequences may not, however, completely correlate with serologically detectable VHa determinants. A strain of rabbits carrying the parental chromosome from which the Basilea mutant was derived has been developed and shown to have a restriction fragment length polymorphism (RFLP) of the K2 gene also found in Basilea rabbits. This allows linkage studies of the K1 phenotype, K2 RFLP and a newly discovered RFLP of the rabbit T cell receptor chain constant region gene. We have analyzed and predicted the locations of kappa light chain allotypic determinants. Predicted determinants were external, located in or near loops, and fell in two clusters of potentially interacting regions within which several overlapping sets of epitopes could occur. Interaction of anti-K1 antibodies with such epitopes on IgG anti-hapten antibodies abolished hapten-mediated dissociation of the antibody from haptgen-coupled cells.