REGULATION OF CELL GROWTH BY TRANSFERRIN RECEPTORS

迄今为止研究的所有细胞都需要转铁蛋白才能生长。 我们和其他人 已经表明转铁蛋白受体的抗体阻断了 淋巴母细胞样细胞系。 在有丝分裂原刺激的淋巴细胞中，这些 抗体阻断增殖。 我们正在研究 调节淋巴细胞中这些受体的出现， 淋巴母细胞样细胞系，以及这些受体在细胞中的功能 生长和新陈代谢。 我们已经证明，G1期阻滞发生在两个 当转铁蛋白受体被阻断时，即使 细胞表达高水平的生长因子受体信使RNA 以及c-myc和c-myb信使RNA。 此外，无论是阻断钙， 通道或向细胞中添加cAMP导致G1期阻滞和细胞周期的丧失。 转铁蛋白受体mRNA cAMP的作用可以在 转录。

All cells studied to date require transferrin for growth. We and others have shown that antibodies to the transferrin receptor block the growth of lymphoblastoid cell lines. In mitogen-stimulated lymphocytes, these antibodies block proliferation. We are studying the processes which regulate the appearance of these receptors in lymphocytes and lymphoblastoid cell lines, and the function of these receptors in cell growth and metabolism. We have demonstrated that G1 arrest occurs in both normal and malignant cells when transferrin receptors are blocked, even if cells are expressing high levels of growth factor receptor messenger RNA and c-myc and c-myb messenger RNA. Furthermore, either blockade of calcium channels or addition of cAMP to cells resuls in G1 arrest and loss of transferrin receptor mRNA. The effect of cAMP can be detected at the level of transcription.