GERM CELL-SPECIFIC MOLECULES OF SPERMATOZOA

针对精子、生精细胞或 分离的抗原正被用来鉴定和表征分子 特定于生殖细胞。正在测试的一般假设是细菌 细胞特异性基因产物负责独特的结构和 精子的功能特征。精子成分正在研究中 它们是：细胞骨架蛋白和表面成分。纤维鞘是 一种细胞骨架结构，位于质膜下面并围绕着 哺乳动物主要部分的外密纤维和轴丝 精子鞭毛。一种能识别一种蛋白的单抗被发现 用2D-SDS-PAGE和Western blotting检测表观MR 70K和pI 8.5 程序。对精子发生过程中的抗原表现进行了分析 16~30日龄小鼠睾丸提取液的酶联免疫吸附试验。反应性 出现在出生后18到20天之间，在时间上与 精子细胞的出现。目前正在测试的假设是 纤维鞘蛋白是生殖细胞特有的中间纤维。 角蛋白家族的蛋白质。精子表面成分的研究包括 附睾期出现在精子表面的一种糖蛋白 成熟。一种单抗已经被用来证明该抗原 由附睾体中的细胞分泌并与血浆结合 鞭毛的膜。该抗体与一种糖蛋白反应 体部和尾部无精子液中存在明显的MR 85K 附睾症。然而，洗涤剂的提取释放出明显的MR 54K 附睾尾部精子中的抗原，表明该成分 在附着在精子上的过程中会发生改变。抗体与蛋白质发生反应。 分离的尾部精子提取液中表观MR的110K、86K和72K 在非变性PAGE条件下，表明84kd的蛋白是 通过二硫键连接到其他质膜相关组分。

Monoclonal antibodies prepared to spermatozoa, spermatogenic cells, or isolated antigens are being used to identify and characterize molecules specific to germ cells. The general hypothesis being tested is that germ cell-specific gene products are responsible for the unique structural and functional characteristics of spermatozoa. Sperm components being studied are: cytoskeletal proteins and surface components. The fibrous sheath is a cytoskeletal structure which underlies the plasma membrane and surrounds the outer dense fibers and axoneme in the principal piece of the mammalian sperm flagellum. A monoclonal antibody was found to recognize a protein of apparent Mr 70 K and pI 8.5 using 2D SDS-PAGE and Western blotting procedures. Antigen appearance during spermatogenesis was analyzed by ELISA on extracts of testes from mice 16 to 30 days of age. Reactivity appeared between 18 and 20 days after birth, temporally coincident with the appearance of spermatids. The hypothesis currently being tested is that the fibrous sheath protein is a germ cell-specific intermediate filament protein of the keratin family. Studies on sperm surface components involve a glycoprotein that appears on the sperm surface during epididymal maturation. A monoclonal antibody has been used to show that the antigen is secreted by cells in the corpus epididymidis and binds to the plasma membrane of the flagellum. The antibody reacts with a glycoprotein of apparent Mr 85 K present in sperm-free fluid from the corpus and cauda epididymidis. However, detergent extraction releases an apparent Mr 54 K antigen from sperm of the cauda epididymidis, suggesting that the component is modified during attachment to sperm. The antibody reacts with proteins of apparent Mr ll0 K, 86 K, and 72 K in cauda sperm extracts separated under non-denaturing PAGE conditions, suggesting that the 84 kd protein is linked by disulfide bonds to other plasma membrane-associated components.