CHARACTERIZATION OF OPIOID RECEPTORS IN BRAIN AND PERIPHERAL TISSUES

我们已经使用定量的配体结合研究来表征 大鼠脑内阿片受体的多种类型和亚型 肾上腺髓质、培养的嗜铬细胞和其他外周组织。 我们已经证明了以前已知的Mu、Delta和 Kappa受体，并存在Mu-L和Mu-2亚型， Kappa-L、Kappa-2、Kappa-3亚型药理独特 配置文件。这是通过使用计算机模拟来实现的 优化实验设计的同时进行实验 多个配体的存在，联合使用药物阻断 以及同时对多个数据集进行计算机建模 适当的约束。

We have used quantitative ligand binding studies to characterize the multiple types and subtypes of opioid receptors in rat brain, bovine adrenal medulla, cultured chromaffin cells, and other peripheral tissues. We have demonstrated the existence of the previously known mu, delta and kappa receptors, and in addition the existence of mu-l and mu-2 subtypes, and of kappa-l, kappa-2, kappa-3 subtypes with distinctive pharmacological profiles. This has been made possible by use of computer simulation to optimize experimental design for experiments involving the simultaneous presence of multiple ligands, the combined use of pharmacological blockade and computer modelling of multiple data sets simultaneously with appropriate constraints.