TRANSGENIC MOUSE MODEL SYSTEM FOR THE ETS-1 AND ETS-2 PROTO-ONCOGENE FUNCTION

研究了转基因表达对造血调控的影响。 在转基因小鼠中检查，其中Ets 2基因由 小鼠金属硫蛋白启动子。 已经发现转基因 表达导致来自骨髓的细胞数量增加 和脾能够在半固体培养基中形成集落，所述半固体培养基含有 IL-3。 转基因小鼠被发现有更高的百分比， 巨核细胞在他们的骨髓比对照组动物。 骨髓 来自转基因动物的培养物表达更高水平的 c-mpl基因与正常对照相比。 c-mpl编码受体 血小板生成素的基因

The effect of transgene expression on the regulation of hematopoiesis was examined in transgenic mice in which the Ets2 gene was driven by the mouse metallothionein promoter. It has been found that transgene expression results in an increase in the number of cells from bone marrow and spleen capable of forming colonies in semisolid media containing IL-3. The transgenic mice were found to have a higher percentage of megakaryocytes in their bone marrow than control animals. Bone marrow cultures derived from transgenic animals express higher levels of the c-mpl gene as compared to normal controls. c-mpl codes for the receptor for the platelet-inducing cytokine, thrombopoietin.