CORE--MOLECULAR RESOURCES

我们建议建立一个分子资源核心实验室（MRCL）， 提供基本试剂、服务和 提供方法方面的援助，以支持 SCOR。 该MRCL设计为集中设施， 仪器，专业的分子生物学专业知识，以及试剂 以使其具有极高的成本效益和高效率。 MRCL 将有助于设计和合成寡核苷酸， 引物、探针和反义试剂。 我们还将提供合成 寡肽和这些肽的位点特异性变体， 免疫原和作为探针在分子解剖的免疫 参与免疫应答或耐受的启动的相互作用。 一些专门的载体，用于克隆和表达选定的 将制备cDNA、限制性内切酶和放射性标记试剂， 存储在核心设施中，并提供给SCOR调查人员。 此外，我们将帮助个人的主要调查人员 在进行一些更专门的实验， 各种cDNA的克隆、表达、位点特异性诱变，以及其它 核酸操作。 最后，MRCL人员将参与 纯化，分子表征和微测序的罕见的 多肽产物（例如参与转录的反式作用蛋白质 胶原基因表达的控制）。 MRCL取代了蛋白质 化学核心作为早期资助的SCOR的一部分， 设想是一个微化学设施，具有更大的范围，以充分 以满足当前的需求。

We propose to establish a Molecular Resources Core Laboratory (MRCL), with the specific purpose of providing essential reagents, services and assistance with methodologies in support of the six subcomponents of the SCOR. This MRCL is designed as a centralized facility for the instrumentation, specialized molecular biology expertise, and the reagents in order to make it extremely cost effective and highly efficient. MRCL will help in the design and synthesis of oligonucleotides to be used as primers, probes and antisense reagents. We will also provide synthetic oligopeptides and site-specific variants of these peptides to be used as immunogens and as probes in the molecular dissection of the immunological interactions involved in the initiation of immune response or tolerance. A number of specialized vectors for cloning and expression of selected cDNAs, restriction enzymes, and radiolabeled reagents will be prepared and stored in the Core facility and made available to the SCOR investigators. In addition, we will help the principal investigators of the individual projects in carrying out some of the more specialized experiments involving cloning, expression, site-specific mutagenesis of various cDNAs, and other nucleic acid manipulations. Finally, MRCL personnel will be involved in purification, molecular characterization and microsequencing of the rare polypeptide products (e.g. transacting proteins involved in transcriptional control of collagen gene expression). The MRCL, which replaces the Protein Chemistry Core established as part of the earlier funded SCOR, is envisioned to be a microchemical facility with much greater scope to fully satisfy the needs of the current SCOR.