BIOREMEDIATION OF SOIL

微生物和植物具有很大的降解潜力， 受污染的有机和金属废物的解毒 环境. 发展有效的就地处理技术的一个障碍是 生物医学系统是我们对生物医学系统的理解 的功能的要求，动态和限制， 自然生态系统中的微生物，如土壤。 这 包括对本土微生物的限制，以及引入 转基因微生物的生长和功能， 植物和植物/微生物系统。 大多数生态学研究的补救措施 微生物已经检查了细菌或真菌的单个分离物;相对而言， 关于微生物聚生体的生态学或 治疗微生物与植物的互利关系。 此外，人们对生物修复的局限性知之甚少， 通常含有一种以上有毒有机化学品的复杂系统 或者重金属 该项目将作为基本的 降解生物的遗传学、生物化学和生理学研究 以及这些研究的应用，特别是生物修复 污染的土壤。 该项目的具体目标包括：1。 确定影响生存和活动的环境因素 降解微生物引入污染土壤; 2. 研究重要的代谢、生态和遗传 影响微生物降解活性的相互作用 3）检测污染土壤和根际土壤中的 土壤重金属对污染土壤细菌种群的影响 4）确定金属和有机物转移的可能性 通过植物吸收进入食物链;和5）调查潜在的 通过植物吸收和转化进行土壤修复。

Microorganisms and plants have great potential for the degradation or detoxification of organic and metallic wastes in contaminated environments. One obstacle to the development of effective in situ bioremedial systems is our fundamental lack of understanding concerning the requirements, dynamics, and limitations on the functioning of degradative microorganisms in natural ecosystems such as soil. This includes limitations on indigenous microorganisms, as well as introduced genetically-modified microorganisms, and on the growth and functioning of plants and plant/microbe systems. Most ecological studies of remedial organisms have examined single isolates of bacteria or fungi; relatively little is known about the ecology of microbial consortia or the mutualistic association of remedial microorganisms with plants. Moreover, little is known about the limitations to bioremediation in complex systems which often contain more than one toxic organic chemical or heavy metal. This Project will act as the interface between basic genetic, biochemical, and physiological studies of degradative organisms and the application of these studies, specifically the bioremediation of contaminated soils. Specific aims of this project include: 1. Determining the environmental factors affecting the survival and activity of degradative microorganisms introduced into contaminated soil; 2. Investigating the important metabolic, ecological, and genetic interactions which influence the degradative activity of microbial consortia in contaminated soil and the rhizosphere; 3) Examining the effect of soil metals on bacterial populations present in contaminated systems; 4) Determining the potential for transfer of metals and organics into the food chain via plant uptake; and 5) Investigating the potential for soil remediation via plant uptake and transformation.