MOLECULAR CLONING OF HUMAN PLATELET GLYCOPROTEIN IB

描述：这些研究的长期目标是了解 Von Willebrand受体GP Ib分子的结构与功能 水平。该受体由4个亚基组成，即GP Ibα、GP Ibβ、GP 剪切力影响GP Ib和von之间的相互作用 Willebrand因子和第一个具体目标是确定是否存在剪切 影响GP Ib和/或von Willebrand因子。在这个特定的环境中进行实验 AIM还将表征参与剪切诱导的结合结构域 可溶性和固定化von Willebrand因子与GP Ib的结合全科医生 IB-von Willebrand因子相互作用导致细胞内变化 第二个具体目标是确定GP的胞内结构域是如何 IB复合体参与了这种跨膜信号转导。GP Ib的作用 β-磷酸化、14-3-3蛋白和肌动蛋白结合蛋白 将对信号进行评估。

DESCRIPTION: The long term goal of these studies is to understand the structure and function of the von Willebrand receptor GP Ib at a molecular level. This receptor consists of 4 subunits, GP Ib alpha, GP Ib beta, GP IX, and GP V. Shear forces affect the interaction between GP Ib and von Willebrand factor and the first specific aim is to determine whether shear affects GP Ib, von Willebrand factor, or both. Experiments in this specific aim will also characterize the binding domains involved in the shear-induced binding of soluble and immobilized von Willebrand factor to GP Ib. The GP Ib-von Willebrand factor interaction leads to intracellular changes and the second specific aim is to determine how the intracellular domains of the GP Ib complex participate in this transmembrane signaling. The role of GP Ib beta phosphorylation, protein 14-3-3, and actin-binding protein in this signaling will be assessed.