NEURAL PROCESSES IN OLFACTORY CODING

描述：本研究建议书中描述了七个实验 嗅球中的神经活动作为刺激编码的基础， 嗅觉系统 初步数据表明，乙酸异戊酯和 丁酸异戊酯激活嗅球肾小球的相同区域 层，但这些区域的嗅球不被激活， 乙酸乙酯或丁酸乙酯。 这一发现表明，异戊基可能是 嗅觉“原始”、“气味型”或“表位”。“实验1将 确定丙酸异戊酯是否也激活这些相同的区域， 将该增味剂的活化模式与丙酸乙酯进行比较。 一 该实验的第二部分将确定分支结构或 酯键的“O-侧”上的烃的数量导致 这些区域的差异激活。 随后的实验解决了 问题是，同样的肾小球被乙酸异戊酯激活， 丁酸异戊酯（Exp. 2);疏水性对 2DG吸收模式（实验3）;碳链长度的影响是什么 2DG灶上的末端官能团（实验4）; 6和8直链脂肪族化合物的末端官能团 碳对2DG摄取模式的影响（实验 5）; 2DG摄取模式 由无气味化合物刺激产生（Exp. （6）;变化 2DG摄取模式作为刺激浓度变化的函数 化合物（Exp. 7）。

DESCRIPTION: Seven experiments are described in this proposal to study neural activity in the olfactory bulb as the basis of stimulus coding in the olfactory system. Preliminary data indicates that isoamyl acetate and isoamyl butyrate activate the same regions of the olfactory bulb glomerular layer, but that these regions of the olfactory bulb are not activated by ethyl acetate of ethyl butyrate. This finding suggests that isoamyl may be an olfactory "primitive," "odotype," or "epitope." Experiment 1 will determine if isoamyl propionate also activates these same regions and the activation pattern by this odorant will be compared to ethyl propionate. A second part of this experiment will determine if the branched structure or number of hydrocarbons on the "O-side" of the ester bond lead to differential activation of these regions. Subsequent experiments address issues such as, is the same glomerulus activated by isoamyl acetate and isoamyl butyrate (Exp. 2) ; what is the influence of hydrophobicity on patterns of 2DG uptake (Exp.3); what is the effect of carbon chain length and terminal functional group on 2DG foci (Exp.4); what is the effect of the terminal functional group of straight-chain alphatic compounds of 6 and 8 carbons on the pattern of 2DG uptake (Exp. 5); 2DG uptake patterns resulting from stimulation with odorless compounds (Exp. 6); and changes in 2DG uptake patterns as a function of changes in concentration of stimulating compounds (Exp. 7).