NOVEL METHOD TO MEASURE BILE ACID TRANSPORT

这是来自BioPhysics Assay Laboratories，Inc.的新I期申请。该公司成立于1997年，拥有四名员工，旨在验证并进一步开发一种测量胆汁酸转运的新方法。该项目将集中在胆酸标记不同的稳定同位素通过两种不同的螯合物。本I期提案中的实验将寻求评价标记效率、总电荷、在温度变化的缓冲液中孵育的稳定性、在竞争性螯合物和血清环境中挑战的稳定性、检测灵敏度和使用分离的肝细胞的生物活性保留。总的目标是开发一种易于使用和成本效益的标记胆汁酸测定试剂盒，适用于研究和临床应用。目前没有执行该功能的常规检测。拟议商业应用：不可用

This is a new Phase I application from BioPhysics Assay Laboratories, Inc. a company founded in 1997 with four employees that proposes to validate and further develop a novel method to measure bile acid transport. The project will focus on cholic acid labeled with different stable isotopes via two different chelates. Experiments in this phase I proposal will seek to evalulate labeling efficiency, overall charge, stability to incubation in buffer with temperature variation, stability to challenge with competing chelates and in a serum environment, sensitivity of detection, and retention of biological activity using isolated hepatocytes. The overall goal is to develop an easy to use and cost effective labeled bile acid assay kit, suitable for research and clinical applications. There is no current routine assay to perform this function. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE