CHROMOSOME SPECEFIC DNA PROBES FOR LABORATORY SPECIES

荧光染色体特异性DNA探针可以识别 间期细胞和有丝分裂细胞中的各种染色体异常 包括非整倍体和断裂/重排事件， 被称为“分子细胞遗传学”的遗传学研究。整个 染色体探针和染色体区域特异性探针可用于 人类和小鼠染色体，但很少有这样的探针可用于大鼠 用于生殖的其他实验室物种的染色体， 发育和癌症研究。我们建议为下列人士提供该等探头： 大鼠、兔和非人灵长类动物染色体的染色体DNA 通过显微解剖和人工细菌的制备和筛选， 染色体（BAC）文库。具体来说，在第二阶段，我们将开发 用于大鼠、兔和恒河猴的染色体特异性点探针， 以及用于兔和恒河猴的一个子集的全染色体涂料探针 染色体兔全染色体涂料探针的选择 恒河猴将通过与经验丰富的合作者协商确定 体内遗传毒理学和生殖生物学。另外我们 建议开发用于大鼠和人类的探针组， 染色体鉴定，无需Giemsa显带或其他 更困难的染色程序。

Fluorescent chromosome-specific DNA probes allow identification of a variety of chromosome abnormalities in interphase and mitotic cells including aneuploids and breakage/rearrangement events, opening a new area of genetic investigation known as "molecular cytogenetics". Whole chromosome probes and chromosome region-specific probes are available for human and mouse chromosomes, but few such probes are available for rat chromosomes of other laboratory species used in reproductive, developmental, and cancer studies. We propose to provide such probes for rat, rabbit and non-human primate chromosomes by chromosomal DNA microdissection and by making and screening bacterial artificial chromosome (BAC) libraries. Specifically, in Phase II we will develop chromosome-specific point probes for the rat, rabbit, and rhesus macaque, and whole chromosome paint probes for a subset of rabbit and rhesus chromosomes. The choice of whole chromosome paint probes for rabbit and rhesus will be determined by consultation with collaborators experienced in in vivo genetic toxicology and reproductive biology. In addition, we propose to develop sets of probes for rat and human which will be used for chromosome identification, without the need for Giemsa banding or other more difficult staining procedures.