REGULATION OF SODIUM, POTASSIUM, CHLORINE AND HYDROGEN TRANSPORT

我们的研究项目集中在对未解决的问题的研究上 离子在根尖膜和基底膜上的传输机制 已定义的小管节段。我们将应用小管灌注和补片- 评价细胞和分子转运机制的钳制技术 Na、K、C_1、H及其生理调节。 首先，我们建议探索近端小管氯化物的机制。 重点是顶端甲酸盐氯化物和草酸盐氯化物的转运 交换。这些研究将针对回收的性质 甲酸盐和草酸盐的作用机理及阴离子的参与 交换剂在近曲小管NACI生理调节中的作用 运输。我们还将研究氯离子通道在 基底膜在介导净氯重吸收中的作用。 其次，我们建议研究Na、K、Ca和H转运的相互作用 在主管细胞中。我们将分析它们的酸碱运移 机制，并探讨它们的调节和与净钠的相互作用 和钾的运输。我们的研究将为我们提供关于 “膜串扰”及其影响Na、K、pH和 CA动态平衡。 第三，我们建议探索心尖低电导K通道。 主管细胞。我们将解决尚未解决的问题 传导机制和生理调节。我们还计划 探索最近克隆的ATP敏感通道并比较其特性 主肾小管上皮细胞具有天然的K通道。

Our research project focuses on the study of unresolved problems of the mechanisms of ion transport across the apical and basolateral membranes of defined tubule segments. We shall apply tubule perfusion and patch- clamp techniques to evaluate cellular and molecular transport mechanisms of Na,K,C1 and H and their physiological regulation. First, we propose to explore the mechanism of proximal tubule chloride transport with emphasis o apical formate-chloride and oxalate-chloride exchange. These studies will be directed at the nature of the recycling mechanisms of formate and oxalate and the participation of the anion exchangers in the physiological regulation of proximal tubule NaCI transport. We shall also investigate the role of chloride channels in the basolateral membrane in mediating net chloride reabsorption. Second, we propose to study the interaction of Na,K,Ca and H transport in principal tubule cells. We shall analyse their acid-base transport mechanisms, and explore their regulation and interaction with net sodium and potassium transport. Our studies will provide insights into "membrane cross-talk" and the signaling pathways affecting Na,K,pH and Ca homeostasis. Third, we propose to explore the apical low-conductance K channel in principal tubule cells. We shall address unresolved problems of conduction mechanism and physiological regulation. We also plan to explore a recently clone ATP-sensitive channel and compare its properties with native K channels in principal tubule cells.