Quantitation of Biomarkers of Oxidative Stress by NICI-MS

1996年举办了一次关于氧化测试的研讨会 NIEHS对人类的压力进行了研究。曾经有一位将军 一致认为开展一项研究来比较 从同一样本测量的各种氧化应激标志物。 一个这样的标记物是F2a-异前列腺素，它由 NICI--女士质谱学工作组已同意 在NIEHS内部进行这些分析。自那以后，我们已经详细阐述了 分析方案，以提高总的成品率和可靠性 样本检查方案，目前正在确定异前列腺素 循环法研究中的水平。其他一些研究涉及 异前列腺素的测定作为氧化应激的指标 也已经启动了。其中一项研究是与NCI合作进行的 (菲尔·泰勒博士)和美国农业部(大卫·贝尔博士和约瑟夫·贾德博士) 女性酒精研究的一部分。这一部分的目的是 我们正在参与的研究是为了确定酒精的影响 对血脂水平、氧化应激和微量营养素的消耗 在绝经后妇女中的水平，并评估 酒精和激素代谢的遗传多态 荷尔蒙水平。第二项研究探讨了最近 预防和干预试验表明， 富含胡萝卜素的食物在疾病早期提供了一种保护 饮食类胡萝卜素晚期干预的优势 会加重癌症。据推测一种抗氧化剂 只有在肿瘤形成的最早阶段的环境才会 最终降低肿瘤的发病率和严重程度； 相反，相同的抗氧化剂方案出现后， 癌前病变会导致后续肿瘤的恶化。 负担。我们将提供氧化的定量测量 包括动物和细胞培养在内的这些研究中的重点 学习。

In 1996 a workshop on Measurement of Oxidative Stress in Humans was held at NIEHS. There was a general consensus that it would be valuable to mount a study comparing various markers of oxidative stress measured from the same sample. One such marker is F2a-isoprostane which is quantitated by NICI-MS. The mass spectrometry workgroup has agreed to undertake these analyses within NIEHS. We have since elaborated the analytical scheme to improve overall yield and reliability of the sample workup protocol, and are currently determining isoprostane levels in the round-robin study. A number of other studies involving measurement of isoprostanes as an indicator of oxidative stress have also been initiated. One such study is a collaboration with NCI (Dr. Phil Taylor) and USDA (Drs. David Baer and Joseph Judd) as part of the Women's Alcohol Study. The aim of the part of the study we are participating in is to determine the effect of alcohol consumption on lipid levels, oxidative stress, and micronutrient levels in postmenopausal women, and to assess the influence of alcohol and hormone metabolism genetic polymorphism on hormone levels. A second study probes questions raised by recent prevention and intervention trials that implied that consumption of carotenoid-rich foods early in disease states provides a protective advantage while late intervention with dietary carotenoids exacerbates cancer. It is hypothesized that an anti- oxidant environment only during the earliest stage of tumorigenesis will ultimately decrease the incidence and severity of tumors; conversely, the same anti-oxidant regimens fed after appearance of preneoplastic foci will lead to exacerbation of the subsequent tumor burden. We will provide quantitative measurement of oxidative stress in these studies which include both animal and cell culture studies.