CORE--MOLECULAR BIOLOGY FACILITY

自从确定了导致囊性纤维化的基因以来 (Cf)，囊性纤维化跨膜传导调节因子(Cftr)， 分子生物学技术的可获得性和使用已成为 在研究这种疾病的分子缺陷方面是必不可少的。这 SCOR致力于研究CFTR的结构和功能，以及 与上皮细胞功能相关的其他离子通道 分泌物。每个研究项目都将利用分子生物学 技巧。Core将提供表达载体等试剂 含有野生型或突变形式的cftr，协助 亚克隆单个通道，如cGMP门控的Na+通道 表达载体、设备和专业知识用于cDNA测序、位点 定向突变和细胞转染法。

Since the identification of the gene responsible for cystic fibrosis (CF), the cystic fibrosis transmembrane conductance regulator (CFTR), the availability and use of molecular biologic techniques has become essential in the study of the molecular defect in this disease. This SCOR is focused on the study of the structure and function of CFTR and upon other ion channels functionally associated with epithelial secretion. Each of the Research Projects will utilize molecular biologic techniques. The Core will provide reagents such as expression vectors containing either wild-type or mutant forms of CFTR, assistance in the subcloning of individual channels such as the cGMP gated Na+ channel into expression vectors, facilities and expertise for cDNA sequencing, site- directed mutagenesis and cell transfection.