CORE--HUMAN CELL AND TISSUE ACQUISITION

建议的研究取决于人肺的可用性。 气管、支气管上皮(TBE)细胞。TbE细胞是成骨细胞的祖细胞 因此，对肺癌的研究至关重要。 城市空气增加支气管性疾病风险的机制 癌症。人类细胞和组织机构的作用是收集 并为建议中的研究提供人体组织和细胞 项目。核心设施主任将与 俄亥俄医学院器官移植团队肯尼斯·克罗普博士， M.D.和生命连接托莱多地区办公室收集 人体肺来自器官捐赠者时，肺不适合 移植。核心设施主任还将与博士们进行互动。 胸外科医生Durham和Durzinsky从 肺癌的手术切除。肺部将被解剖并 上皮细胞和支气管灌洗液恢复正常 具体项目。在一些研究中，气管和支气管组织将 固定在甲醛中，然后染色评估有丝分裂和凋亡 分数。人类细胞和组织资源设施将使我们能够满足 项目调查人员对维护的组织和细胞的需求 在标准化条件下对多学科类型至关重要 这项提案中概述的研究。核心将承担起责任 与器官移植团队互动并分发组织 并将细胞送往各个实验室。提供的组织将是 附有年龄、性别、种族、城市或农村居住地的数据， 职业，吸烟。向项目2提供的材料将包括；单元 作为解剖学函数的衍生、有丝分裂和凋亡部分 在气管支气管树中的位置。为项目3提供的服务将 包括：测量肺细胞中多环芳烃DNA和组蛋白加合物的TbE细胞 (Lafleur和Giese)和支气管灌洗液和巨噬细胞 粒子角色化(Vander Sande)。核心的具体目标 建议1.收集和分发细胞、组织和灌洗液 从人体实质和呼吸道中提取，以供项目研究人员使用； 确保组织和组织的统一活性和适当的特征 细胞，3.使用以前开发的技术来解剖和 定量分离呼吸道上皮细胞 解剖人肺，4.与项目主任合作，协调 利用核心进行个人实验，并协助其他研究 依靠人类肺组织或细胞的需要而努力。

The proposed studies depend on the availability of human lung tracheobronchial epithelial (TBE) cells. TBE cells are the progenitors of bronchogenic carcinoma and therefore are crucial to the study of mechanisms by which urban air increases the risk for bronchogenic carcinoma. The role of the Human Cell and Tissue Facility is to collect and dispense human tissues and cells for the studies in the proposed projects. The Core Facility Director will interact with the Director of the Medical College of Ohio Organ Transplant Team, Dr. Kenneth Kropp, M.D., and the Toledo regional office of Life Connection to collect whole human lungs from organ donors when the lungs are not suitable for transplantation. The Core Facility Director also will interact with Drs. Durham and Durzinsky, thoracic surgeons, to obtain lung specimens from surgical resections to bronchogenic carcinoma. Lungs will be dissected and epithelial cells and bronchial lavage fluid recovered as required for specific projects. For some of the studies, tracheo-bronchial tissues will be fixed in formaldehyde, then stained to assess mitotic and apoptotic fractions. A Human Cell and Tissue ore Facility will enable us to meet the needs of project investigators for tissues and cells that are maintained under standardized conditions essential for the types of multidisciplinary studies outlined in this proposal. The Core will have the responsibility of interacting with the organ transplant team and distributing the tissue and cells to the various laboratories. The tissue provided will be accompanied by data on age, sex, race, urban or rural residence, occupation, smoking. Material provided to project 2 will include; cells derived and mitotic and apoptotic fractions as a function of anatomical position in the tracheobronchial tree. Services provided to project 3 will include; TBE cells to measure PAH DNA and histone adducts in lung cells (Lafleur and Giese) and bronchial lavage fluid and macrophages for particle characterization (vander Sande). The specific aims of the core proposal are to 1. collect and distribute cells, tissues, and lavage fluid derived from human parenchyma and airways to project investigators, 2. assure uniform viability and appropriate characterization of tissues and cells, 3. use previously developed techniques to dissect and quantitatively recover epithelial cells separately from airways of dissected human lungs, 4. work with the Program Director to coordinate the individual experiments using the Core as well as assist other research efforts relying on the need for human lung tissues or cells.