CORE--ANALYTICAL CORE

在多个调查员、多个项目计划项目中， 需要开展科学和行政支助活动。 这些最好是以集中方式提供，而不是 尝试根据特定项目对它们进行分段 和/或调查员，特别是当有许多领域 重叠。支持核心提供了这样的集中化方法 并基于两个组件。分析组件将 为各个项目提供支持，为结构、 药物和代谢物的定性和定量分析。燃气 将进行层析/质谱分析 通过使用稳定的同位素而具有高度的敏感性和特异性 结合电子捕获的稀释法 负化学电离。液相色谱/电喷雾 电离串联质谱仪将用于 药物代谢物的鉴定。交替质谱学 使用电子电离、正极化学的方法学 电离，液体二次离子，基质辅助激光解吸 如有必要，可用。该组件还将提供 合成药物代谢物和稳定同位素的资源 用作稳定同位素稀释分析标准的类似物。 需要常规高效液相色谱的分析方法 如果需要，将在核心中开发方法 个别项目。一个管理组件将提供 支持基本但非科学相关的结构 单个项目的活动。例如，管理 协调、财务管理、用品订购、手稿 和赠款报告的编写，以及提供共同 复印、服务合同、邮寄和旅行等服务 支持等。此外，对所有 项目将由核心提供。

In a multi-investigator, multi-project Program Project, a number of scientific and administrative support activities are required. These are best provided in a centralized fashion rather than attempting to fragment them according to a particular project and/or investigator, especially when there are many areas of overlap. The Support Core provides such centralized approaches and is based on two components. The analytical component will provide support to the various Projects for the structural, qualitative and quantitative analysis of drugs and metabolites. Gas chromatography/mass spectrometry analyses will be performed with high sensitivity an specificity by the use of stable isotope dilution methodology in combination with electron capture negative chemical ionization. Liquid chromatography/electrospray ionization tandem mass spectrometry will be employed for the identification of drug metabolites. Alternative mass spectrometry methodology that employs electron ionization, positive chemical ionization, liquid secondary ion, matrix-assisted laser desorption is available should it be required. This component will also provide a resource for the synthesis of drug metabolites and stable isotope analogs for use as standards for stable isotope dilution assays. Analytical procedures that require conventional HPLC methodology will be developed in the Core if they are required in the individual projects. An administrative component will provide the structure to support essential but non-scientifically related activities for the individual Projects. For example, administrative coordination, fiscal management, ordering of supplies, manuscript and grant-report preparation, and the provision of common services such as duplicating, services contracts, mailing and travel support, etc. In addition, biostatistical support for all of the Projects will be provided by the Core.