RAPID IDENTIFICATION OF CLINICALLY SIGNIFICANT AIRBORNE FUNGAL SPORES

该项目的长期目标是确定丰度 大气中空气传播的真菌孢子，并获得 对孢子发育的机制、孢子的理解 存活率和孢子萌发影响临床发病率 对人类健康有重要影响的空气传播真菌孢子 过敏障碍、感染和霉菌毒素等问题。这个 具体目标是(1)改善和扩大颗粒大小 基于分析仪的孢子快速识别方法，(2)增加了 关于临床上和临床上的大气丰度的信息库 与大气发生有关的萌发。《安徒生》 大气采样器将按月计划使用，以收集 并对它们进行粒度分析。为了获得更好的 对孢子发育、孢子存活和 相差扫描、荧光扫描的孢子萌发技术 将使用电子显微镜和透射电子显微镜观察 该真菌野生型和突变型的某些关键时期， 鸡腿菇(Coprinus Cinereus)由于其担孢子发育同步， 实验室操作的简便性和生产的无性 和有性孢子，灰曲霉将被用作建立 “造物主”事件在发展、生存和萌芽中。突变株 将用于突出关键阶段。枝孢霉，一种常见的 采集到大量的真菌，而曲霉菌却不多见 丰富的真菌将与灰霉菌一起进行研究。这些真菌 将暴露在模拟的环境压力下并分析 细胞水平上的微扰。知道在多大程度上 环境和内在因素对气传真菌孢子的影响 流行率将为准确估计真菌提供手段 大气中的病原体。

The long-term objectives of the project is to determine the abundance of airborne fungal spores within the atmosphere and gain an understanding of the mechanism by which spore development, spore survival and spore germination influence prevalence of clinically significant airborne fungal spores that are responsible human health problems such as allergic disorders, infections and mycotoxins. The specific objectives are (1) improve and expand the particle size analyzer-based rapid spore identification method, (2) increase the information bank about the atmospheric abundance of clinically and germination relevant to atmospheric occurrence. The Andersen atmospheric sampler will be used on a monthly schedule, to collect spores and subject them to a particle size analysis. To obtain a better understanding of the extent that spore development, spore survival and spore germination techniques of phase contrast, florescent scanning electron and transmission electron microscopies will be used to observe certain critical stages in wild type and mutant strain of the fungus, Coprinus cinereus. Because of its synchronous basidiospore development, ease of manipulation in the laboratory and production of both asexual and sexual spores, C. Cinereus will be used as a model for establishing "maker" events in development, survival and germination. Mutant strains will be used to highlight critical stages. Cladosporium, a frequently collected and abundant fungus, and Aspergillus a frequent but not abundant fungus will be studied along with C. Cinereus. These fungi will be exposed to simulated environmental stresses and analyzed perturbations at the cellular level. Knowing the extent to which environmental and intrinsic factors influence airborne fungal spore prevalence will provide the means for accurate estimates of fungal disease agents in the atmosphere.