CORE--STRUCTURAL DATA FROM NMR SPECTROSCOPY

描述：这个核心项目将提供一些成员的核磁共振数据 一组表达的流感嗜血杆菌蛋白将由其他 项目的组件。将在早期阶段使用核磁共振进行筛选 溶解度条件(在低聚集状态下)，以及程度 蛋白质的折叠。当找到合适的条件时，标记为 将产生蛋白质，并确定共振分配 采用多核多维方法。有了骨干任务， 可以对二级结构进行快速评估，这将有助于 投入到结构预测工作中。对核磁共振数据的进一步分析将 然后导致对褶皱(低分辨率结构)的识别，以及 可在需要时按下以提供高分辨率结构。这个 努力将由Organ博士指导，并主要由One Research执行 助理(Sari博士)，大约1/2的600 MHz光谱仪 为工作干杯。预计罪魁祸首将是2个低分辨率的数量级 每年1个高分辨率构造。

DESCRIPTION: This core project will provide NMR data on some members of the set of expressed H. influenzae proteins that will be provided by other components of the project. NMR will be used in early stages to screen conditions for solubility (in states of low aggregation), and the degree of folding of the protein. When suitable conditions are found, labeled protein will be generated and resonance assignments will be determined using multi-nuclear multi-dimensional methods. With backbone assignments, a quick assessment of secondary structure can be made, which will feed into the structure prediction effort. Further analysis of NMR data will then lead to identification of the fold (a low resolution structure), and can be pushed on to give high resolution structure when desired. The effort will be guided by Dr. Organ, and largely executed by one Research Associate (Dr. Sari), with about 1/2 of a 600 MHz spectrometer dedicated to the work. The expected culprit will be the order of 2 low resolution and 1 high-resolution structure per year.