CORE--MORPHOLOGY

这个核心是一个中央形态设施，为 项目中的调查员。 这包括光学显微镜， 透射电子显微镜、免疫荧光和 免疫组织化学处理和分析，如有说明， 扫描电子显微镜，形态分析，摄影和 图形插图服务。 根据我们以前的经验， 显微镜、透射电子显微镜和免疫组织化学 将是调查人员使用最多的服务。 在此 在过去的一年里，有1,500多个样本被处理用于光学显微镜检查。 分析，预计需求将持续旺盛。 为这项服务的所有部门的计划项目。 比如说， 在第I、II、III和IV节中，将常规使用光学显微镜检查， 监测各种模型中肺损伤的强度。 在 此外，分离细胞和组织培养的体外研究 第I、II、III和V部分中的单层将利用该技术。 透射电子显微镜（TEM）也将被广泛使用 特别是在第一节，第二节和第四节， 评估体内内皮和上皮细胞损伤， 第II、III和V节将评估肉芽肿的变化 通过这种方法。 TEM也用于组织的形态测定分析 改变。 扫描电子显微镜（SEM）将用于 选择的病例主要是为了评估牙槽骨表面的损伤， I节中粘附的白细胞。 冰冻切片分析 尤其是免疫组织化学， 调查人员目前正在使用，目前将继续使用 提议 免疫组织化学是评价肿瘤的主要技术 粘附分子和细胞因子在体内和体外的上调， 在第I、II、III和V节中广泛用于此目的。 将在体内进行选定的免疫荧光研究，以评价 免疫复合物诱导的损伤及其对干预研究的影响 并在体外评估单核细胞与内皮细胞的结合， 第五节.形态测定分析现在经常进行， 量化体内肺损伤的强度和 通过测量参数如肉芽肿大小， 白细胞流入、出血和实质细胞损伤，包括 和上皮细胞。 最后，核心设施定期提供 为该计划的调查人员提供摄影和制图服务 项目包括海报和出版物图形的准备。

This core is a central morphology facility providing support for the investigators in the program project. This includes light microscopy, transmission electron microscopy, immunofluorescence and immunohistochemistry processing and analysis plus where indicated scanning electron microscopy, morphometric analysis, photographic and graphic illustration services. Based on our previous experience light microscopy, transmission electron microscopy and immunohistochemistry will be the services most heavily utilized by investigators. During this past year, over 1,500 samples were processed for light microscopy analysis and it is anticipated that there will be continue heavy demand for this service by all sections of the program project. For example, in section I, II, III, and IV light microscopy will be routinely used to monitor the intensity of the lung injury in the various models. In addition, in vitro studies of isolated cells and tissue culture monolayers in section I, II, III, and V will utilize this technique. Transmission electron microscopy (TEM) will also be utilized extensively for this program project particularly in sections I,II, and IV for assessment of injury to endothelial and epithelial cells in vivo and sections II, III and V where alterations in granulomas will be assessed by this method. TEM also is used for morphometric analysis of tissue alterations. Scanning electron microscopy (SEM) will be utilized in selected cases primarily to evaluate injury to the alveolar surface of the lung by adherent leukocytes in section I. Frozen section analysis of tissues and cells, particularly immunohistochemistry, is heavily utilized currently by investigators and this will continue in the current proposal. Immunohistochemistry is the primary technique for evaluating adhesion molecule and cytokine upregulation in vivo and in vitro and will be used extensively for this purpose in section I, II, III, and V. Selected immunofluorescence studies will be done in vivo to evaluate immune complex induced injury and the effects on interventional studies and in vitro to assess binding of monocytes to endothelial cells in section V. Morphometric analysis now routinely done to precisely quantify the intensity of lung injury in vivo and the effects of therapeutic interventions by measuring parameters such as granuloma size, leukocyte influx, hemorrhage and injury to parenchymal cells including and epithelial cells. Finally, the core facility routinely offers photographic and graphic services for investigators in the program project including preparations of poster and publication graphics.