Development of an Embryo Cryopreservation Program in Laboratory Animals

NIH动物遗传资源（NIHAGR） 维持着几个动物物种和许多宝贵的基因型 在一个物种中，它们被用作生物医学研究的模型。 为了保护这一种质，建立了胚胎库， 无限期保存这些原种和菌株的经济手段 啮齿类动物和兔子。胚胎冷冻保存有其他 基因型管理的好处，如防止损失 从疾病，生殖失败，自然 灾难或基因污染。冷冻库最小化 通过提供一种资源， 定期重新推导定义的基因型， 基础繁殖殖民地。作为公认的存储库， 实验室动物物种，NIHAGR常规提供育种 核在整个生物医学研究界的要求。 冷冻保存的胚胎代表了 一种更简单、更人道、更容易的运输方法 全世界的遗传物质。胚胎的另一个重要影响 处理、冷冻保存和转移程序是为了消除 某些鼠科疾病的垂直传播基因型和 物种差异继续影响这一效率， 努力(This是校内研究项目的延续 Z01-RR-00077-13 VR.）

The NIH Animal Genetic Resource (NIHAGR) maintains several animal species, and many invaluable genotypes within a species, which are used as models in biomedical research. To protect this germ plasm, an embryo bank was established as an economical means to indefinitely preserve these stocks and strains of rodents and rabbits. Cryopreservation of embryos has other benefits for genotype management such as safeguarding against loss of germ plasm from disease, reproductive failure, natural catastrophe or genetic contamination. The cryobank minimizes changes due to genetic drift by providing a resource from which to periodically rederive defined genotypes for restandardization of foundation breeding colonies. As a recognized repository for laboratory animal species, the NIHAGR routinely provides breeding nuclei upon request throughout the biomedical research community. Rather than shipping live animals, cryopreserved embryos represent a simpler, more humane and easier method to transport requested germ plasm worldwide. Another important impact of embryo handling, cryopreservation, and transfer procedures is to eliminate the vertical transmission of some murine diseases. Genotype and species differences continue to impact on the efficiency of this is effort. (This is a continuation of Intramural Research Project Z01-RR-00077-13 VR.)