IN VITRO MUTAGENESIS OF CHICKEN HISTONE & HMG GENES

HMG-14和HMG-17是普遍存在的非组蛋白染色体蛋白 与核小体核心颗粒结合 他们组成了一个家庭， 已报道结合活性染色质的染色体蛋白质。 为了了解这些蛋白质的体内功能，我们破坏了 HMG-14 a和HMG-17两者在禽类B细胞系DT 40中。 我们的结果 表明敲除细胞的表型没有明显变化 相对于亲本DT 40细胞。 此外，没有补偿 在细胞中观察到HMG-14 b或组蛋白水平的变化 缺乏HMG-14 a和HMG-17。 二维凝胶电泳 分析发现，一种主要表达的蛋白质被激活， 敲除细胞 开发分析凝胶中蛋白质的方法， 直接在转移膜上被用来识别这一点 特别的地方。

HMG-14 and HMG-17 are ubiquitous non-histone chromosomal proteins that bind to nucleosome core particles. They form a family of chromosomal proteins that have been reported to bind active chromatin. To understand the in vivo function of these proteins, we disrupted both HMG-14a and HMG-17 in an avian B-cell line DT40. Our results indicate that the knockout cells show no obvious change in phenotype with respect to the parental DT40 cells. Furthermore, no compensatory changes in HMG-14b or histone protein levels were observed in cells lacking both HMG-14a and HMG-17. Upon 2-D gel electrophoretic analysis, a major expresssed protein was found to be activated in the knockout cells. Developing methods for analyzing proteins in gels and directly on transfer membranes are being employed to identify this particular spot.