HYDROLYTIC CLEAVAGE OF RNA BY METAL COMPLEXES, FUNDAMENTAL STUDY IN CATALYSIS

金属催化的RNA的水解性裂解是 基因表达的化学。例如，成熟度和 RNA的降解是由核糖核酸酶和核酶在 几乎总是需要金属辅因的工艺。然而， 这些金属的详细化学作用或作用在很大程度上仍不清楚。 我们打算揭开金属离子在这些反应中的作用 利用这些机械信息设计了一系列综合的 核酶。在我们进行机械研究的同时，我们还采用了 合成类比方法的一种变体，其中泛函 设计并制备了核糖核酸酶的模拟物。在这里，我们到了 水解性合成核酶的研究进展 金属络合物。质谱学被用来表征这些 材料。

The metal-catalyzed hydrolytic cleavage of RNA is fundamental to the chemistry of gene expression. For example, the maturation and degradation of RNA are carried out by ribonucleases and ribozymes in processes that nearly always require metal cofactors. However, the detailed chemical role or roles of the metals remain largely unknown. We intend to unravel the role of metal ions in these reactions and utilize this mechanistic information to design a series of synthetic ribozymes. In parallel with our mechanistic studies, we are employing a variant of the synthetic analogue approach in which functional mimics of RNA enzymes are designed and prepared. Here, we are developing synthetic ribozymes derived from hydrolytically active metal complexes. Mass spectrometry is used for characterizing these materials.