PORPHYRIN NUCLEIC ACID INTERACTIONS

我们已经制备了含有烷基卟啉的杂化化合物。 通过氧丙基连接物嵌入到寡脱氧核苷酸中 主链的磷酸基团。这些都使我们能够研究 结构相似的一种卟啉之间的相互作用 辅因子；存在于自然界和DNA中。(以前在这个领域的工作有 几乎完全是用异种生物做的 中-四芳基卟啉)。不同温度下的一系列核磁共振波谱 记录序列5‘-CGCGCCTTC-P-CATTGCGG-3’的温度， 其中-P-表示与互补DNA络合的卟啉 在面对卟啉的部位带有胸苷残基的链。 这套复式公寓被选为最稳定的一套 不同的复合体。在FBML的500 MHz上获取的光谱 光谱仪使我们能够得出定性的结构 关于卟啉-DNA相互作用的信息。与其他人一起 光谱和足迹数据，依赖于温度的核磁共振 数据使我们能够提出大周期的疝间插入法。 作为卟啉-DNA最有可能的结构结果 互动。

We have prepared hybrids containing an alkylporphyrin covalently embedded in oligodeoxynucleotides via oxypropyl linkers to the phosphate groups of the backbone. These have allowed us to study interactions between a porphyrin similar in structure to common cofactors; found in nature and DNA. (Previous work in this field has almost exclusively been performed with xenobiotic meso-tetraarylporphyrins). A series of NMR spectra at different temperatures were recorded of the sequence 5'-CGCGCCTTC-P-CATTGCGG-3', where -P- denotes the porphyrin, complexed with a complementary DNA strand bearing a thymidine residue at the site facing the porphyrin. This duplex had been selected as the most stable among a number of different complexes. The spectra acquired on the FBML's 500 MHz spectrometer have allowed us to derive qualitative structural information about the porphyrin-DNA interactions. Together with other spectroscopic and footprinting data, the temperature-dependent NMR data have allowed us to propose herniintercalation of the macrocycle as the most likely structural consequence of the porphyrin-DNA interactions.