NITROGLYCERIN TOLERANCE DEVELOPMENT & RECOVERY IN LLC PK1 CELLS

我使用计算机图形学实验室的设施有很多 药代动力学、药效学、扩散和最优控制 模拟。我们目前涉及两个主要领域： 研究：1)最优控制理论应用于给药 2)GTN诱导的cGMP耐受性的定位 动力学(包括发展和恢复空间)。我们已经选择了 耐受性的不同药代动力学/药效学(PK/PD)模型 并对这些模型进行了仿真。我们还运行了最优 模拟/计算。最优曲面描述最优的 对于某些给定的目标函数，给药方案可以最好地描述 通过2-D和3-D绘图。这项工作是使用交互式 CGL中的图形工作站。现在我们又增加了一个数学模型 模型到公差模型阵列。这一模式仍在继续 基于对LLC-PK1细胞的实验工作和 GTN。我们已经得到了一些关于动力学的实验结果。 该细胞系的耐受性发展。我们正在积极地 对这一动力学过程进行数学建模，并进行模拟 也是。CGL资源允许我们进行模拟和图形化 我们实验数据的映射，以及与 理论上产生的动能空间。

I use the Computer Graphics Laboratory facilities for many pharmacokinetic, pharmacodynamic, diffusion, and optimal control simulations. We are currently involved in two major areas of research: 1) optimal control theory applied to dosing drugs that exhibit tolerance and 2) the mapping of the GTN-induced cGMP tolerance kinetics (both development and recovery space). We have selected different pharmacokinetic/pharmacodynamic (PK/PD) models for tolerance and simulated these models. We have also run optimal simulations/calculations. The optimal surfaces describing an optimal dosing regimen for some given objective function can best be described by 2-D and 3-D plots. This work is done using the interactive graphics workstations in CGL. Now we are adding another mathematical model to the array of tolerance models. This model is still being developed and is based upon experimental work with LLC-PK1 cells and GTN. We have obtained some experimental results regarding the kinetic development of tolerance in this cell line. We are actively mathematically modeling this kinetic process and are doing simulations too. CGL resources allow us to do both the simulations and graphical mapping of our experimental data, as well as visual comparisions to the theoretically generated kinetic space.