RATIONAL CHARACTER OF PROTEIN CRYSTAL QUALITY HIGH RESOLUTION DATA COLLECTION

我们的目标是开发非主观的方法来评估蛋白质 并使用这些方法来客观地评价晶体的完好性 并比较了蛋白质晶体生长的各种方法和数据 收集。我们的结果将应用于比较微重力 与地球晶体生长和低温数据的比较 收集方法。该提案包含一项综合计划，以 蛋白质晶体质量的全面表征及晶体关联 晶体形态、生长条件和低温数据的质量 收集。对晶体质量的测量将包括地形， 摇摆宽度马赛克测量和标准高分辨率数据 收集。我们将研究蛋白质晶体的完美性 满足以下条件：(1)不同的晶体 相同蛋白质的形态可用，(2)微重力 生长的样品是可用的，(3)低温条件已经 已经成立了。

Our goal is to develop nonsubjective methods to evaluate protein crystal perfection and to use these methods to to objectively evaluate and compare various methods of protein crystal growth and data collection. Our results will be applied to comparing microgravity versus earth-bound crystal growth and to comparing cryogenic data collection methods. This proposal contains an integrated program to fully characterize protein crystal quality and to correlate crystal quality with crystal morphology, growth conditions and cryogenic data collection. Measurements of crystal quality will include topography, rocking-width mosaicity measurements and standard high-resolution data collection. We will examine protein crystal perfection where the following conditions have been met: (1) different crystal morphologies are available for the same protein, (2) microgravity grown samples are available and (3) cryogenic conditions have been established.