BACTERIAL RUVB DNA HELICASE

我们计划进行一些试点试验，以收集低价 一个(或多个，取决于时间)晶体上的分辨率反射 细菌RuvB DNA解旋酶的形式。解旋酶形成一种 具有32点群对称性和多个晶体的六面体环 形式具有实质上的非晶体对称性。我们希望尝试一下 使用一些使用非常低分辨率的相移技术 反射与非晶体对称性相结合 平均。SSRL的数据收集结果将用于 与阶段化模型相结合，该模型将基于以下条件生成 从电子角度测量解旋酶环的尺寸 显微镜或从电子显微镜中观察晶体本身 与斯克里普斯研究所的马克·耶格尔博士合作。

We plan to conduct some pilot experiments to collect the low resolution reflections on one (or more, depending on time) crystal forms of the bacterial RuvB DNA helicase. The helicase forms a hexameric ring with 32 point group symmetry and a number of crystal forms have substantial non-crystallographic symmetry. We hope to try to use some of the phasing techniques that use very low resolution reflections in combination with non-crystallographic symmetry averaging. The results of the data collection at SSRL will be used in combination with phasing models that will either be generated based on measurements of the dimensions of the helicase ring from electron microscopy or from electron microscopy of the crystals themselves in collaboration with Dr. Mark Yeager at The Scripps Research Institute.