SPIROCHETE MORPHOLOGY BY 3 D RECONSTRUCTION

(受NIAID R01 AI29743、R29 AI35401、NIDR K11 DE00257至 螺旋体是一组细菌，具有独特的 形态学。最外面是一个膜鞘，在这个鞘内是 螺旋形的电池圆柱体。在电池圆柱体的每一端， 细菌鞭毛样细胞器，称为周质鞭毛 附在这里。这些周质鞭毛位于 外膜鞘和细胞圆柱体。基因分析显示 这些细胞器参与了螺旋体的运动。在……里面 此外，有几条证据表明这些周质 鞭毛推动生物体旋转的方式类似于 细菌鞭毛。我们的研究重点集中在 准确地确定这些螺旋体是如何游泳的。我们知道， 周质鞭毛不仅在运动中起作用，而且还 在它们所在的域中影响单元的形状。我们 用HVEM立体摄像机和倾斜系列胶片来确定 生物体内周质鞭毛的形态：如何 它们缠绕在细胞圆柱体上，以及在什么方向上，即 顺时针或逆时针。结果帮助我们确定了 这些细胞器如何影响细胞的形状和运动性 有机体。四年前，我们录制了一盘录像带 为戈尔茨坦博士拍摄的倾斜系列电影。从那时起，我们就获得了 改进了软件。来自三个倾斜系列的图像被重新扫描 并重新调整，并制作了三部新的和改进的电影。一种新的 制作了录像带，其中包括彩色标题板和 在每个倾斜系列之前插入的低放大静止视图。

(Supported by NIAID R01 AI29743, R29 AI35401, NIDR K11 DE00257 to N. Charon) Spirochetes are a group of bacteria with a unique morphology. Outermost is a membrane sheath, and within this sheath is a helical-shaped cell cylinder. At each end of the cell cylinder, bacterial flagella-like organelles, called periplasmic flagella, are attached. These periplasmic flagella reside in the space between the outer membrane sheath and cell cylinder. Genetic analysis has shown that these organelles are involved in the motility of spirochetes. In addition, several lines of evidence indicate that these periplasmic flagella propel the organisms by rotation in a manner similar to bacterial flagella. The thrust of our research is centered on determining exactly how these spirochetes swim. We know that the periplasmic flagella not only play a role in motility, but also influence the shape of the cell in the domain where they reside. We used HVEM stereopairs and tilt-series mo vies to de termine the configuration of the periplasmic flagella within the organism: how they wrap around the cell cylinder, and in what direction, i.e. clockwise or counter-clockwise. The results helped us to determine how these organelles influence the shape and the motility of the organisms. Four years ago, we made a video tape of several tilt-series movies for Dr. Goldstein. Since then we have acquired improved software. The images from three tilt series were rescanned and realigned, and three new and improved movies were made. A new videotape was made which included color title panels and low-magnification still views inserted before each tilt series.