SINGLE MOLECULE FLUORESCENCE MICROSCOPY METHODS

微观技术已经被开发出来，以将 与时间相关的单光子计数。对以下内容进行重大修改 共焦装置允许同时进行两种颜色监视 荧光标签以及探测光束的偏振调制。 此外，许多数学算法也被纳入其中 进入数据分析并进一步探索，以帮助 单分子发射的解释，它们的时间 相关关系及其与构象变化的关系 这些组件。核糖体翻译研究，GCN-4 多肽构象动力学和构象动力学 正在进行集光组装(见亮点)。

Microscopic techniques have been developed to incorporate time-correlated single photon counting. Significant modifications to the confocal apparatus allow for simultaneous two color monitoring of fluorescent tags as well as polarization modulation of probe beams. In addition, numerous mathematical algorithms are being incorporated into the data analysis and further explored to aid in the interpretation of the single molecule emissions, their time correlations and the relationship of them to conformational changes in these assemblies. Investigations into ribosomal translation, GCN-4 peptide conformational dynamics as well as conformational dynamics in light harvesting assemblies are being undertaken (see Highlights).