PHOTOCHEMISTRY & STRAINED MOLECULES

酵母菌的液泡与哺乳动物的液泡有许多共同特征 溶酶体 它是一个酸性的隔间，包含几个主要的 水解酶，d是几种化合物的最终目的地， 通过受体介导的内吞作用和液相进入细胞 内吞作用 因为酵母可以遗传，生化， 和细胞生物学分析，因为液泡是如此之大， 酵母细胞中的一个明显的细胞器，酵母是一个很好的模型 系统的功能和组装的研究这个细胞器。 这个实验室收集了大量的 液泡功能和组装。 分子生物学的进步 使我们能够很容易地克隆和测序基因所确定的 有缺陷的突变体 仅在1988/89年，我们就克隆和测序了4个 基因（PEP 3，PEP 5，PEP 7，PEP 12）被发现参与液泡 功能 掌握了序列后，我们转向计算机分析 到目前为止，我们一直依赖于现在的服务， 已不存在的生物网络。 我们具体的计算机需求是：1）排序 管理2）核酸和氨基酸相似性搜索， 3）一级和二级结构分析。 大部分 我们的需求可以通过加载在VAX上的威斯康星州软件包（UWGCG）来满足。 为了节省时间，CRAY将用于比较我们的蛋白质 序列与EMBL或GENBANK的所有六个翻译的阅读框 使用FASTDB的数据库。 我们与PSC互动的设施包括 带有2400波特调制解调器的IBM-PC，它允许我们访问Telenet 公共数据网络或拨号进入卡内基梅隆大学 Annex终端服务器和连接到 卡内基梅隆大学通过AppleTalk连接器上网。 我们没有 目前的财政或超级计算支持我们的计算需求

The vacuole of yeast shares many features with mammalian lysosomes. It is an acidic compartment, contains several major hydrolases, an d is the final destination for several compounds which enter the cell by receptor-mediated endocytosis and fluid-phase endocytosis. Because yeast is amendable to genetical, biochemical, and cell biological analysis, and because the vacuole is such a large and conspicuous organelle in a yeast cell, yeast is an excellent model system for studies on the function and assembly of this organelle. This lab has accumulated a large collection of mutants defective in vacuolar function and assembly. Advancements in molecular biology has allowed us to easily clone and sequence the genes identified by the defective mutants. In 1988/89 alone we have cloned and sequenced 4 genes (PEP3, PEP5, PEP7, PEP12) found to be involved in vacuolar function. With sequence in hand we have turned to computer analysis for which up till now we have relied on the services of the now defunct BIONET. Our specific computer needs are: 1) sequencing management 2) nucleic and amino acid similarity searches and comparisons and 3) primary and secondary structure analysis. Most of our needs can be met by the Wisconsin package (UWGCG) loaded on VAX. In the interest of time the CRAY would be used to compare our protein sequences to all six translated reading frames of EMBL or GENBANK databases using FASTDB. Our facilities for interacting with PSC are an IBM-PC with a 2400 baud modem which allows us to access the Telenet Public Data Network or to dial into the Carnegie Mellon University Annex Terminal Server and a Macintosh which is connected to the Carnegie Mellon Internet through an AppleTalk connector. We have no current financial or supercomputing support for our computing needs