MR MICROSCOPY OF FIXED MOUSE EMBRYOS

妊娠8.5至19.5天的小鼠胚胎将被 在9.4特斯拉系统上进行了研究。 我们将测试 胚胎的预处理技术，以确定什么固定 技术和什么反应混合物提供了最有用的信息 每个胚胎阶段。 具体来说，我们将确定 渗透作用和与铁盐和亚铁盐的反应有助于 11.5 ~ 14.5天神经嵴结构增强 小鼠胚胎 我们也会寻找增强信号的来源 在发育中的骨骼中，如在14.5天之间的胚胎中所证明的， 出生 我们还将扫描已经灌注了 Gd-BSA-DTPA完成我们的心血管时间序列研究 正常胚胎的发育。

Mouse embryos between 8.5 and 19.5 days of gestation will be investigated on the 9.4 Tesla system. We will be testing pre-processing techniques of the embryos to determine what fixation techniques and what reaction mixes provide the most useful information for each embryo stage. Specifically, we will determine whether permeabalization and reaction with ferric and ferrous salts contribute to enhanced contrast of neural crest structures in 11.5 to 14.5 day mouse embryos. We will also search for the source of enhanced signal in developing bone as demonstrated in embryos between 14.5 days and birth. We will also scan embryos which have been perfused with Gd-BSA-DTPA to complete our time-series study of cardiovascular development in the normal embryo.