MODELING OF NEUROTRANSMITTER GATED CHANNELS, RECEPTORS, PROTEINS: STRUC & FUNCT

这个项目的总体目标是在分子水平上研究 水平，结构和功能之间的相关性 神经递质门控通道、受体和相关蛋白质。 目前，该实验室的主要工作重点是对 结构域和结构域的二级结构、拓扑和功能作用 同源重组人α1甘氨酸的特异性残基 受体在杆状病毒表达系统中过表达。这 抑制性门控阴离子通道是配基门控的一员 超家族，包括同系物烟碱乙酰胆碱 受体，5-羟色胺5-羟色胺受体和GABA受体，都是 它们在突触的信号转导中起着快速的中介作用。 这些调查可能会提供对一般保护的洞察 渠道设计中使用的机构。与之相关的类似研究 NMDA型谷氨酸受体与促性腺激素释放激素 (GnRH)受体也将被传导。这些受体也是 大家庭的成员，他们的结构特征可能是 NMDA受体是兴奋性的一员。 谷氨酸受体家族参与突触传递和 兴奋性毒性，GnRH受体是大G 蛋白偶联受体，是调节的关键作用 神经和内分泌系统的协调。此外, 锌金属内肽酶EP 24.15的结晶轨迹 降解性激素释放激素，也在进行中。

The overall goal of this project is to examine, at a molecular level, the correlation between structure and function for several neurotransmitter-gated channels, receptors, and related proteins. Currently, the main focus of the laboratory is the examination of the secondary structure, topology, and functional role of domains and specific residues of the homomeric recombinant human $alph$1 glycine receptor overexpressed in a baculovirus expression system. This inhibitory gated anionic channel is a member of the ligand-gated superfamily, which include the homologous nicotinic acetylcholine receptor, the 5-HT3 serotonin receptor, and the GABA receptor, all of which act in rapid mediation of signal transduction at the synapse. These investigations may provide insight into the general conserved mechanism used in channel design. Analogous studies concerning the NMDA type glumatmate receptors and the gonadotropin releasing hormones (GnRH) receptor will also be conducted. These receptors, too, are members of large families, and may their structural featur may be paradigmatic; the NMDA receptor is a member of the excitatory glutamate receptor family which act in synaptic transmission and excitotoxicity, and the GnRH receptor is a member of the large G protein coupled receptors and is a pivotal role in regulating coordination of the neural and endocrine systems. In addition, crystalliszation trails of EP 24.15 a zinc metalloendopeptidase which degrades GnRH, are also underway.