RAT GLIOMA LACTATE TURNOVER: MEASURED BY IN VIVO NMR SPECTROSCOP: BRAIN NEOPLASM

在过去的两年里，我们专注于探索和建立 ~(13)C宽带质子去耦合的可行性及评价 光谱学在4特斯拉。一种新颖的线圈设计，使我们能够 在低于1.5特斯拉的功率水平时解耦是 介绍并演示了我们在人体肌肉中检测到的 来自天然丰富的谷氨酸的窄共振(2-4赫兹线宽)， 肌酸、胆碱和牛磺酸。我们建立了完整的三维 基于极化转移的~(13)C-MRS局域化研究 体内选择的定位化质子激发碳-13图像 准确地说，是光谱学。我们展示了成功的 4T条件下人脑的三维定位 消除所有头皮脂肪信号，并记录有显著意义 与以前相比，4T(~5倍)的灵敏度增加 报告为2.1特斯拉。代谢物峰的线宽为 在人脑中低至2-3赫兹，本质上与 那些报告在较低的田地。~(13)C-MRS也用于联合 用~1H MRS将一个异常高的峰指定给肌醇 通过检测到74.5ppm的高强度来检测正常的人脑。

In the past two years, we focused on exploring and establishing the feasibility of broadband proton decoupling and evaluating 13C spectroscopy at 4 Tesla. A novel coil design, that has allowed us to decouple at power levels below those reported at 1.5 Tesla was introduced and we demonstrated in human muscle, the detection of narrow resonances (2-4 Hz linewidth) from natural abundance glutamate, creatine, choline, and taurine. We established full three-dimensional localization of 13C MRS using polarization transfer based on which we dubbed Proton Excited Carbon-13 Image Selected in vivo Localized spectroscopy, PRECISELY. We demonstrated the successful three-dimensional localization in the human brain at 4T via the elimination of all scalp lipid signals and documented significant sensitivity increases at 4T (~ factor 5) over those previously reported at 2.1 Tesla. Metabolite peaks had linewidths that were as low as 2-3 Hz in the human brain, which is essentially the same as those reported at lower fields. 13C MRS was also used in conjunction with 1H MRS to assign an unusually high peak to scyllo-inositol in the normal human brain by detecting an elevated intensity at 74.5 ppm.