FLUORIMETRIC CHARACTERIZATION OF RETROVIRAL PEPTIDES: HIV

肌钙蛋白复合物（肌钙蛋白T（TnT）、肌钙蛋白I（TnI）、肌钙蛋白C （TnC））在钙依赖性调节细胞凋亡中起重要作用。 肌肉收缩。 为了研究的邻近关系的 肌钙蛋白T-I-C亚基、TnT的一系列额外突变和25 制备了TnT的K片段，在大肠杆菌中表达并纯化。 需要TnT的单抽头突变体K片段（TnT/25 K W265 F）， 供体通过荧光能量转移进行距离测量。 选择了一系列供体-受体对， 在不同缓冲条件下测量的性质。 的距离 TnT/K的Tap-212与Tin-Cys-96（N96 C）、Tin-Cys-117（S117 C）之间， 测定TnC-Met-25和TnC-Cys-98。 距离测量 正被用于开发肌肉收缩的模型。

Troponin complex (troponin T (TnT), troponin I (TnI), troponin C (TnC)) plays an important role in the Ca2+ -dependent regulation of muscle contraction. To study the proximity relationships of the troponin T-I-C subunits, a series of additional mutation of TnT and 25 K fragment of TnT have been made, expressed in E.coli and purified. The single-Tap mutant K fragment of TnT (TnT/25 K W265F) was needed as the donor for distance measurements by fluorescence energy transfer. Series of donor-acceptor pairs were chosen and the spectroscopic properties measured under different buffer conditions. The distances between Tap-212 of TnT/K to Tin-Cys-96 (N96C), Tin-Cys-117 (S117C), TnC-Met-25 and TnC-Cys-98 were measured. The distance measurements are being used to develop models for muscle contraction.