WASTE SITE ASSESSMENT

描述：重建项目的目标是发展一个 评估与以下物质有关的人类健康风险的综合程序 超级基金网站。生物测试将提供数据来定义 免疫毒性、遗传毒性、发育和神经毒性潜力 环境样本。将进行五项实验，作为 这个项目是为了评估一种基于生物的方案的实用性 在超级基金网站评估人类健康风险。前两个 实验将评估与复杂混合物相关的风险 超级基金站点以及模型化合物和重组混合物 AH型和HAH型化学品的代表。实验1设计为 要比较人类健康风险的估计值，请使用生物学或 化学分析。这项实验将涉及收集 来自超级基金网站和匹配的控制区的样本。环境 样本将从超级基金的广泛网站收集 氢氧化物代表(即炼油废物、煤气化废物)和 HHA(即木材保护废物、多氯联苯污染的土壤和沉积物) 污染物。将使用以下方法对现场样本进行初始筛选 一次急性毒性试验和两次微生物遗传毒性试验。已选择 样品也将接受定量的GC/MS分析。基座 根据初步筛选的结果，有限数量的样本 将在开发的毒理学测试电池中进行分析 项目1至6。将进行第二次实验，以 从选定的络合物中分离并分离出最有毒的成分 混合物。将重新组合孤立的组件以进行测试(如 单独的化合物和混合物)，以评估 复杂混合物各组分之间的潜在相互作用。数据 这些实验将被用来提供生物测试数据 描述复杂混合物中各组分之间的相互作用 它们的潜在毒性当量因子(TEF)。第三次和第四次 实验将集中在监测复杂混合物的解毒。 这些实验将提供信息来定义 减少毒性和对人类的潜在威胁的补救技术 环境中复杂混合物的健康。在第五个实验中， 将对层状硅酸盐粘土(天然和化学改性)进行评估 因为它们具有紧密结合和解毒目标化学物质的能力。这个 化学吸附的位置和分子机制将被阐明。

Description: The objective of the renewal project is to develop a comprehensive procedure to assess the human health risk associated with a Superfund site. Biological testing will provide data to define the immunotoxic, genotoxic, developmental and neurotoxic potential of environmental samples. Five experiments will be conducted as part of this project to evaluate the utility of a biologically-based protocol to estimate human health risk at a Superfund site. The first two experiments will assess the risk associated with complex mixtures from Superfund sites as well as model compounds and reconstituted mixtures representative of the AH and HAH chemicals. Experiment 1 is designed to compare estimates of human health risk using either biological or chemical analysis. This experiment will involve the collection of samples from Superfund sites and matched control areas. Environmental samples will be collected from a broad range of Superfund sites representative of AH (i.e. refinery waste, coal gasification waste) and HAH (i.e. wood-preserving waste, PCB contaminated soils and sediments) contaminants. An initial screen of site samples will be performed using an acute toxicity and two microbial genotoxicity assays. Selected samples will also be subjected to a quantitative GC/MS analysis. Based on the results of the preliminary screen, a limited number of samples will be analyzed in the battery of toxicological tests developed in Projects 1 through 6. A second experiment will be conducted to fractionate and isolate the most toxic components from selected complex mixtures. Isolated components will be reconstituted for testing (as individual compounds and mixtures) in the bioassays to evaluate the potential interactions of the components of a complex mixture. The data from these experiments will be used to provide biological test data describing the interactions of the components of complex mixtures and their potential toxic equivalency factors (TEFs). The third and fourth experiments will focus on monitoring detoxification of complex mixtures. These experiments will provide information to define the ability of remedial techniques to reduce toxicity and the potential threat to human health of complex mixtures in the environment. In the fifth experiment, phyllosilicate clays (natural and chemically-modified) will be evaluated for their abilities to tightly bind and detoxify target chemicals. The sites and molecular mechanisms of chemisorption will be elucidated.