Upgrade of Andor Dragonfly for Four Dimensional Cellular Medicine and Super-resolution microscopy

在这里，我们正在申请升级Andor Dragonfly旋转圆盘共聚焦/超分辨率激光扫描显微镜。我们在2016/17年对Andor Dragonfly进行了Beta测试，我们的系统是欧洲第一个。在过去6年中，它为发表22多篇论文作出了重大贡献。在随后的六年里，它被使用了这么多（每年超过1000小时），以至于围绕尼康显微镜支架建造的支架和sCMOS相机需要更换。该系统在过去6年中进行了超过6000小时的研究，是先进成像核心设施中最繁忙的机器。自从Dragonfly最初安装以来，Andor和Nikon都发布了新产品，使新Dragonfly系统更适合我们的研究。我们收到了一个未使用的尼康Ti 2支架，需要安装硬件自动对焦，激光和新物镜。自动饮水机、新型硅胶浸没物镜和新型高灵敏度相机（索纳）将提供无与伦比的景深和视野，以促进类器官、模式生物和器官型的高分辨率实时成像。为确保该系统得到良好的支持，它将被安置在一个专门的实时成像套件中，并由专家技术人员提供支持。我们将在一个全面的服务合同下保持系统。我们相信，随着升级，Dragonfly将继续成为我们MRC HGU研究的基石。蜻蜓是爱丁堡超分辨率联盟（ESRIC）的一部分。

Here we are applying for an upgrade for the Andor Dragonfly spinning disk confocal/ super-resolution laser scanning microscope. We Beta-tested the Andor Dragonfly in 2016/17 and our system was the first in Europe. In the past 6 years it has made a significant contribution to the publication of over 22 papers. It has been used so much (>1000 hours per annum) in the ensuing six years that the stand and sCMOS camera, built around a Nikon microscope stand requires replacement. The system has carried out over 6000 hours of research in the past 6 years and is the busiest machine in the advanced imaging core facility. Since Dragonfly's original installation both Andor and Nikon have released new products that make the new Dragonfly system an even better fit to our research. We have been gifted an unused Nikon Ti2 stand which needs a Hardware autofocus, a laser and new objectives to be fitted. An automatic water dispenser, new silicone immersion objective and new highly sensitive camera (SoNa) will provide unparalleled depth of field and field of view to facilitate high resolution live imaging of organoids, model organisms and organotypics. To ensure that the system is well supported it will be housed in a dedicated live imaging suite and supported by expert technicians. We will keep the system under an all inclusive service contract. We are confident with the upgrades the Dragonfly will continue to be the cornerstone of our MRC HGU research. The Dragonfly is open access as part of the Edinburgh Super-resolution Consortium (ESRIC).