State-of-the-art cell pulsatile physiological stretch & live cell imaging system

在类似于身体正常生理学的环境中研究细胞是可取的。在布里斯托大学，我们有多个研究小组，他们对肾脏，心血管，组织工程和癌细胞/类器官生物学感兴趣。我们已经确定，在一个能够模拟心输出量（幅度和频率）的动态脉动拉伸系统中培养细胞对这些群体非常有益。我们想购买一个最先进的拉伸系统，可以在培养箱中同时拉伸多个细胞/类器官/组织样本数天至数周（与体内情况相匹配）。此外，它还能够通过位于培养箱中的显微镜对标本进行录像，并通过荧光标记评估蛋白质在其细胞位置中的变化。该设备将通过连接到我们专门的沃尔夫森成像中心，提供给大学的所有研究人员。重要的是，目前没有其他设备可以在我们的大学，所以这个设备将促进未满足的需求。

Studying cells in an environment that resembles normal physiology experienced in the body is desirable. In the University of Bristol we have multiple research groups who are interested in kidney, cardiovascular, tissue engineering and cancer cell/organoid biology. We have identified that growing cells in a system that allows dynamic pulsatile stretch that would mimic cardiac output (amplitude and frequency) would be highly beneficial for these groups.We would like to purchase a state-of-the-art stretch system that can simultaneously stretch multiple cell/organoid/tissue samples in an incubator for days to weeks (matching the situation in the body). Furthermore, it has the capability to video the specimens through an incubator located microscope and assess how proteins change in their cellular location through florescent tags.This equipment will be made available to all researchers in the University by being linked to our dedicated Wolfson Imaging Centre. Importantly, there is currently no other equipment that can do this in our University, so this equipment would facilitate an unmet need.