RHIZOBIAL LIPOPOLYSACCHARIDES ESSENTIAL FOR INFECTION

描述：此修订后的应用程序是为了支持调查 LPS多糖组分的结构和生物合成 参与Rhyzobial共生的分子。这些细胞壁多糖 被认为是毒力的重要决定因素， 入侵宿主的可能性总体目标是确定 结构和生物合成的这些复杂的糖脂，并确定 其生物活性所需的特定结构特征。的 具体目标是：1）表征最小lipidA核心结构 O-链多糖连接的要求，和 表征对这种最小脂质的修饰。核心结构 这导致O链多糖的连接增加，2） 表征O-链的结构修饰， 共生感染; 3）分离R.豆科植物， 无法添加LPS中常见的独特结构特征， （4）对该种的R.豆类特异酶 将Kdo 2-lipid-IVA前体加工成这种独特的结构， 有机体

DESCRIPTION: This revised application is to support the investigation of the structure and biosynthesis of the polysaccharide components of the LPS molecules involved in Rhyzobial symbiosis. These cell wall polysaccharides are thought to be important determinants of virulence and to be required for the invasion of the host. The overall goal is to determine the structure and biosynthesis of these complex glycolipids and determine the specific structural features required for their biological activity. The specific aims are: 1) characterize the minimum lipidA core structure requirements for the ligation of the O-chain polysaccharide, and characterize the modification to this minimal lipid. A core structure which lead to the ligation of increased O-chain polysaccharide, 2) to characterize the structural modification to the O-chain that occur during the symbiotic infections, 3) to isolate mutants of R. leguminosarum that are unable to add unique structural features normal found in the LPS of this species, and 4) to characterize the R. leguminosarum specific enzymes that process the Kdo2-lipid-IVA precursor to the unique structure of this organism.