MICROFILAMENTS IN THE YEAST SACCHAROMYCES CEREVISIAE

拟议研究的目标是了解分子的细节。 负责通过以下途径运输和定位分泌性产品 肌动蛋白细胞骨架。目前的研究结果表明，分泌囊泡 由肌球蛋白V沿肌动蛋白电缆向下运输。 实验探索改变的突变体的运输是如何受到影响的 极性以及肌动蛋白细胞骨架的哪些特征是必需的 使其作为肌球蛋白V底物发挥作用。四个具体目标是 介绍：(1)开发场地本地化系统 产生分泌性产品；(2)产生温度敏感型 原肌球蛋白(Tpm1)突变体将用于研究极化 肌动蛋白细胞骨架和分泌过程的去极化； (3)筛选具有tpm1敏感分泌缺陷的突变体； 和(4)鉴定与肌球蛋白V尾巴相互作用的蛋白质 生物化学和遗传方法。

The goal of the proposed studies is to understand the molecular details responsible for the transport and targeting of secretory products via the actin cytoskeleton. Current results suggest that secretory vesicles are transported down actin cables by a myosin V. The proposed experiments explore how transport is affected in mutants with altered polarity and what features of the actin cytoskeleton are required to allow it to function as a myosin V substrate. Four specific aims are presented: (1) to develop a system for the localization of the sites of emergence of secretory products; (2) to produce temperature sensitive tropomyosin (tpm1) mutants that will be used to investigate polarization and depolarization of the actin cytoskeleton and secretory processes; (3) to screen for mutant that have a tpm1 sensitive secretory defect; and (4) to identify proteins that interact with the myosin V tail by biochemical and genetic approaches.