MICROTUBLES AND REGULATION OF FOCAL CONTRACTS

在1994年至1996年由福格蒂基金资助的研究中，我们 建立了病灶细胞骨架调控机制的模型 培养的成纤维细胞中的粘连[FA]。根据这个模型， FA的发展经历了萌芽和成熟两个阶段，每个阶段 阶段由不同的微管依赖的重组控制 肌动蛋白皮质。这个项目的目的是找出 这种机制不仅在极化的成纤维细胞中控制FA，而且 也存在于另一种主要组织类型的细胞中，非极化的 上皮细胞具有完全不同的组织结构 细胞骨架和FA。我们的假设是，类似的基本机制 导致在这两种细胞类型中发展出非常不同的系统 由于微管系统的不同。FA和OF的动力学 细胞骨架将通过免疫化学和免疫- 组织化学方法和干涉反射显微镜； 大脑皮层张力将通过 美国PI实验室。依赖于Rho的收缩能力将被调制 通过一系列的激活剂和抑制剂。改变微管的步骤 系统，我们将使用特定的药物和注射抗体来对抗 微管运动蛋白，运动蛋白。

In the study supported by the Fogarty grant from 1994 to 1996, we developed a model for mechanisms of cytoskeletal control of focal adhesions [FA] in cultured fibroblasts. According to this model, development of FA has two stages, initiation and maturation, and each stage is controlled by different microtubule-dependent reorganizations of the actin cortex. The aim of this project is to find out whether this mechanism controls FA not only in the polarized fibroblasts but also in the cells of another main tissue type, non-polarized epitheliocytes with profoundly different organization of the cytoskeleton and FA. Our hypothesis is that similar basic mechanisms lead to development of very different systems in these two cell types due to the differences of microtubular systems. Dynamics of FA and of the cytoskeleton will be assessed by immunochemical and immuno- histochemical methods and by interference-reflection microscopy; cortical tensions will be assessed by the methods developed in the laboratory of the US PI. Rho-dependent contractility will be modulated by a series of activators and inhibitors. To alter the microtubular system, we will use specific drugs and injections of antibodies against the microtubular motor protein, kinesin.