STRUCTURE AND FUNCTION OF THE COILED COIL MOTIF

描述：(改编自申请人的摘要)中心目标 这项建议将有助于加深对 蛋白质-蛋白质相互作用的物理基础。主题是盘绕线圈 该提案特别强调结构性决定因素 在这一重要而常见的类别中形成同源二聚体和异源二聚体 蛋白质-蛋白质复合体。将采用一系列技术 包括x射线结晶学、计算方法、热力学分析 和基因筛查。该提案试图描述埋葬的角色的特征 描述线圈-线圈相互作用的特异性的极性相互作用； 推广一种新开发的侧链重新包装的计算算法 为了有效地探索影响 合作的热力学；进行序列的数据库分析 与开发算法的最终目标合作的决定因素 将忠实地揭示这种相互作用；并测试和扩展现有的 通过分析基因组中的配对来了解盘绕-螺旋相互作用 水平使用酵母为基础的实验系统。

DESCRIPTION: (adapted from the applicant's abstract) The central objective of this proposal is to contribute to the development of an understanding of the physical basis for protein-protein interactions. Coiled-coils are the subject of the proposal, which has a particular emphasis on the structural determinants of homodimer and heterodimer formation amongst this important and common class of protein-protein complexes. An array of techniques is to be employed including x-ray crystallography, computational methods, thermodynamic analysis and genetic screens. The proposal seeks to characterize the role of buried polar interactions in delineating the specificity of coiled-coil interactions; to extend a recently developed computational algorithm for sidechain repacking in an effort to efficiently explore the parameters that affect the thermodynamics of partnering; to undertake a database analysis of sequence determinants of partnering with an ultimate aim of developing algorithms that will faithfully reveal such interactions; and to test and extend existing knowledge about coiled-coil interactions by analyzing partnering at a genomic level using a yeast-based experimental system.