RAPID SCREENING OF HUMAN GROWTH HORMONE

本提案中描述的研究详细说明了使用免疫测定结合表面增强拉曼散射（Sers）光谱检测和定量人生长激素（HGH）的程序。由于Sers的使用，该技术具有高灵敏度的优点。此外，Sers独特的表面敏感检测意味着可以在不洗掉指示剂抗体的情况下读取测定。这使得可以容易地使该方法自动化，以微孔板形式快速筛选大量样品。该测定将用用Sers活性涂层修饰的微孔板来证明。Sers表面将用抗HGH处理。这些将代表待开发的商业产品的一部分，并将进行耐久性测试。 通过将样品引入改良微孔板，然后加入染料抗HGH结合物进行分析。 只有那些被捕获的HGH捕获在表面上的缀合物才会出现在微孔板的Sers光谱中。我们将使用633 nm HeNe和852 nm二极管激光系统进行比较分析。 将使用人血清进行测试，以显示天然产物的荧光对分析的影响。建议的商业应用该产品将彻底改变人类生长激素的检测，因此它将在临床实验室，制药实验室和研究实验室的应用。此外，该方法可适用于除人生长激素以外的测定。

The research described in this proposal details a procedure for detecting and quantifying Human Growth Hormone (HGH) using an immunoassay coupled with surface enhanced Raman scattering (SERS) spectroscopy. The technique has the advantage of high sensitivity due to the use of SERS. In addition, the unique surface sensitive detection of SERS means that the assay can be read without washing away the indicator antibodies. This makes it possible to easily automate this method for rapid screening of large numbers of samples in a microwell plate format. This assays will be demonstrated with microwell plates modified with a SERS active coating. The SERS surface will be treated with anti-HGH. These will represent part of the commercial product to be developed and will be tested for durability. The analysis will be performed by sample introduction onto the modified microwell plate, followed by addition of a dye antiHGH conjugate. Only those conjugates that are captured on the surface by captured HGH will show up in the SERS spectra of the microwell plates. We will use both 633 nm HeNe and 852 nm Diode laser systems for the analysis for comparison. Tests will be made with human serum to show the affect of fluorescence from natural products on the analysis. PROPOSED COMMERCIAL APPLICATION This product will revolutionize detection of human growth hormone, therefore it will have applications in clinical laboratories, pharmaceutical laboratories, and research laboratories. Additionally, the methodology can be adapted to assays other than that of human growth hormone.