MOLECULAR GENETICS OF VARICELLA ZOSTER VIRUS

水痘-带状疱疹病毒（VZV）引起水痘和带状疱疹。的目标 构建VZV突变体以鉴定VZV基因， 在病毒生长和潜伏期中很重要，为了测试这些 突变体可作为候选活病毒疫苗， 动物与变种人 VZV糖蛋白I基因的失活导致了一种 对细胞的吸附和在组织中的生长受损 培养细胞。 另一个VZV基因ORF 9A的失活降低了VZV的表达。 病毒在体外形成融合细胞（合胞体）的能力。 VZV ORF 66基因的失活表明，该基因产物是 对修饰（磷酸化）蛋白质很重要。 将报告基因（β-半乳糖苷酶）插入VZV基因组中 豚鼠眼内接种表达VZV的VZV， 报告基因 这些动物出现慢性眼部感染（葡萄膜炎） 眼睛里有炎症细胞 报告基因在大肠杆菌中表达。 眼睛的各个部分，包括视网膜，长达3个月后 感染

Varicella-zoster virus (VZV) causes chickenpox and shingles. The goals of this project are to construct VZV mutants to identify VZV genes that are important in virus growth and latency, and to test whether these mutants could serve as candidate live virus vaccines by inoculating animals with the mutants. Inactivation of the VZV glycoprotein I gene resulted in a virus that was impaired for adsorption to cells and impaired for growth in tissue culture cells. Inactivation of another VZV gene, ORF9A, reduced the ability of the virus to form fused cells (syncytia) in vitro. Inactivation of the VZV ORF66 gene showed that this gene product was important for modifying (phosphorylating) proteins. A reporter gene (beta-galactosidase) was inserted into the VZV genome and guinea pigs were inoculated intraocularly with VZV expressing the reporter gene. The animals developed a chronic eye infection (uveitis) with inflammatory cells in the eye. The reporter gene was expressed in various parts of the eye, including the retina, up to 3 months after infection.