ADVERSE EFFECTS OF OXIDATIVE STRESS DURING PREGNANCY

工作总结：氧化损伤与病理有关 几种疾病，但直到最近才有人建议有一种 在不利的生殖条件下发挥作用。许多环境和 职业暴露是已知的促氧化剂(例如，许多溶剂和 金属)，它们可能会引起氧化，从而对生殖产生不利影响 损坏。这项研究项目探索了 环境暴露、氧化应激和不利生殖 条件。 我们的工作有两个主要目标。首先是对飞行员的数据进行分析 将解决抗-HBs标记物的变异量的研究 氧化剂和氧化剂状态由季节性和个体内变化引起 和储存程序，并比较怀孕和 未怀孕的女性。到目前为止的调查结果表明，样品不能 在不改变水平的情况下储存超过几小时 谷胱甘肽和过氧化脂质含量显著升高。第二个目标是工作 与实验室科学家共同设计了一项研究，以评估乳癌患者 氧化状态在流行病学研究中的适用性。这项工作是 去年秋天在NIEHS举行的国际会议的延续 调查了氧化应激标志物的领域。此外，分析 保加利亚关于工业的现有数据的小样本 污染物、氧化损伤的标志物和生殖结果 孕妇和她们的婴儿仍在继续。

Summary of Work: Oxidative damage has been implicated in the pathology of several diseases, but only recently has it been suggested to have a role in adverse reproductive conditions. Many environmental and occupational exposures are known pro-oxidants (e.g., many solvents and metals), and they may adversely affect reproduction by causing oxidative damage. This research project explores the relationship between environmental exposures, oxidative stress, and adverse reproductive conditions. We working with two major aims. First is analysis of data from a pilot study that will resolve the amount of variability in the markers of anti- oxidant and oxidant status due to seasonal and intra-individual variation and to storage procedures, and to compare the results in pregnant and non-pregnant women. Findings to date indicate that samples cannot be stored at all for more than a few hours without altering the level of glutathione and lipid peroxides significantly. A second aim is to work with laboratory scientists in designing a study to evaluate mamkers of oxidative status for suitability in epidemiologic studies. This work is a continuation of an international meeting held last fall at NIEHS that surveyed the field of oxidative stress markers. In addition, the analysis of small samples of existing data from Bulgaria concerning industrial pollutants, markers of oxidative damage, and reproductive outcome in pregnant women and their infants continues.