ULTRAVIOLET RADIATION INDUCED SKIN CANCER

工作总结：UV-B辐射可能会抑制诱导， 诱发迟发型超敏反应（DTH）。 我们已经开发出一种 使用激光多普勒灌注成像分析（DPI）的定量方法 UV-AB诱导的DTH抑制使用的激发反应， 以前结核菌素致敏的个人，以调查 UV剂量与抑制诱导之间的剂量反应关系 或对UV诱导的抑制的抗性。 19名健康志愿者 每天在上背部的划定区域照射四天 UV-AB辐射。调整UV-AB剂量水平以递送60或100 μ g/ml。 120 mJ/cm 2 UV-B辐射。 在第四天，一个照射，一个 每个紫外线剂量的未辐照试验部位随机选择， 并皮内注射结核菌素。 测试反应 在96 h临床上和DPI下进行分析和定量。 的 结果表明，UV-AB辐射对诱导期有抑制作用 结核菌素DTH的剂量依赖性和个体 紫外线辐射的免疫抑制作用的差异。 以低 剂量的UV-B，敏感和抗性表型都观察到， 1：1的比例。然而，加倍UV-B增加了抑制在一些 个体，在其他耐药个体中诱导抑制，或 在其他敏感的植物中诱导抗性或耐受性， 低剂量紫外线。 我们假设紫外线诱导的阈值 抑制DTH和诱导T抑制细胞， 启发差异很大。 因此，UVR的表征 和诱导易感性的UVR剂量反应关系，或 必须确定耐药性，以充分表型为这些 性状

Summary of Work: UV-B radiation may suppresses the induction and elicitation of delayed-type hypersensitivity (DTH). We have developed a quantitative method using laser Doppler perfusion imaging analysis (DPI) of UV-AB induced suppression of DTH using the elicitation response in previously tuberculin sensitized individuals in order to investigate the dose response relationship between UV dose and induction of suppression or resistance to UV induced suppression. Nineteen healthy volunteers were irradiated daily for four days on delineated areas on the upper back with UV-AB radiation. UV-AB dose levels were adjusted to deliver 60 or 120 mJ/cm2 UV-B radiation. On the fourth day, one irradiated and one unirradiated test site for each UV-dose were chosen at random on each individual and injected intradermally with tuberculin. Test reactions were analyzed and quantified at 96 h clinically and with DPI. The results indicate that UV-AB radiation can suppress the elicitation phase of tuberculin DTH and that there are dose dependent and individual differences in the immune suppressive effect of the UV-radiation. At low dose UV-B, both the susceptible and resistant phenotypes were observed at an ratio of 1:1. However, doubling UV-B increased the suppression in some individuals, induced suppression in others that were resistant, or induced a resistance or tolerance in others that were susceptible at the lower dose of UV. We hypothesize that the thresholds for UV induced suppression of DTH and the induction of T suppressor cells that may block elicitation vary considerably. Thus, characterization of both the UVR and the UVR dose response relationship for induction of susceptibility or resistance must be determined in order to adequately phenotype for these traits.