ROLE OF POU DOMAIN GENES IN EARLY VERTEBRATE DEVELOPMENT

POU转录因子已被证明在 调控发育过程中细胞命运的决定。我们已经专注于 关于POU结构域基因XlPOU-2功能的早期认识 神经发育。要在非洲爪哇中生成函数的“敲除”，一个 由增强型阻遏子或VP16激活子组成的结构是 融合到XlPOU 2的POU域。此构造将位于 调节地塞米松并将其注射入A1 血统，一个将成为未来大脑的血统。因此，两者 “显性-否定”构式的时空表达 应该能让我们在早期评估XlPOU2的功能 神经发生。为了了解随后发生的一连串事件 神经诱导，一种克隆上调基因的减法方案 或被神经诱导因子下调，noggin正在进行中。 几个新的候选基因已经被识别出来，并正在进行 特色化的。我们还克隆了一部小说并对其进行了表征 斑马鱼太极中与脑1相关的POU基因。太极是太极的标志 成体硬骨神经的增殖、未分化细胞 系统。人和小鼠太极的特征及其在运动中的作用 神经干细胞将使用原代祖细胞培养进行检测 小鼠的神经元和基因消融。

POU transcription factors have been shown to be important in the regulation of cell fate decisions during development. We have focused on understanding the function of one POU domain gene, XlPOU 2 , in early neural development. To generate "knockout" of function in Xenopus, a construct consisting of the engrailed repressor or VP16 activator was fused to the POU domain of XlPOU 2. This construct will be under the regulation of the dexamethasone and will be injected into the A1 lineage, a lineage that will become the future brain. Thus, both temporal and spatial expression of the "dominant-negative" construct should enable us to assess the function of XlPOU 2 in early neurogenesis. In order to understand the cascade of events that follow neural induction, a subtraction scheme to clone genes that up-regulated or down-regulated by the neural induction factor, noggin is underway. Several novel candidate genes have been identified and are being characterized. We have also cloned and characterized a novel Brain-1-related POU gene in zebrafish, tai-ji. Taiji is a hallmark of proliferating, non-differentiated cells of the adult teleost nervous system. The characterization of human and mouse tai-ji and its role in neural stem cells will be examined using primary progenitor cultures of neurons and gene ablation in the mouse.