NEUROGENESIS AND GLIOGENESIS IN THE DEVELOPING BRAIN

检测培养的星形胶质细胞支持神经元的能力。 在培养中的生存能力。人胎神经元在培养中仍可存活 与人胎儿星形胶质细胞共培养8-10周。这些 细胞产生神经生长因子(NGF)和白介素6(IL-6) 6)。对它们产生其他神经营养因子的能力的研究 作为脑源性神经营养因子和神经胶质源性 神经营养因子(GDNF)正在进行中。的表型特征 胎儿神经元提示，来自星形胶质细胞的支持有助于其 文化中的长寿和活跃度。活体实验目前正在进行中 在啮齿动物和非人灵长类动物的研究中。 我们还鉴定了培养物中正在测试的细胞聚集体 对表皮生长因子(EGF)呈阳性反应。这些细胞可以 代表神经干细胞，既可以自我更新，也可以产生 献给祖先。

Astrocyte cultures were examined for their ability to support neuronal viability in culture. Human fetal neurons remained viable in culture for 8-10 weeks if co-cultivated with human fetal astrocytes. These cells produced nerve growth factor (NGF) as well as interleukin 6 (IL- 6). Investigations of their ability to produce other neurotrophins such as brain-derived neurotrophic factor (BDNF) and glial-derived neurotrophic factor (GDNF) are ongoing. Phenotypic characteristics of fetal neurons suggest that support from astrocytes contributes to their longevity and activity in culture. In vivo experiments are now underway in rodent and nonhuman primate studies. We have also identified aggregates of cells in culture that are testing positive and respond to epidermal growth factor (EGF). These cells may represent neural stem cells which can self-renew as well as give rise to progenitors.