NEURAL PROCESSES IN OLFACTORY CODING

描述：在这份研究计划中描述了七个实验 嗅球的神经活动作为刺激编码的基础 嗅觉系统。初步数据显示，乙酸异戊酯和 丁酸异戊酯激活嗅球肾小球的相同区域 但嗅球的这些区域不是被激活的 丁酸乙酯的乙酸乙酯。这一发现表明异戊基可能是 嗅觉的“原始”、“普通类型”或“表位”。实验1将 确定丙酸异戊酯是否也激活这些相同的区域和 这种气味的激活模式将与丙酸乙酯进行比较。一个 本实验的第二部分将确定分支结构或 酯键的O-侧上的碳氢化合物的数量导致 这些区域的不同激活。后续实验地址 例如，是同一个肾小球被乙酸异戊酯激活和 丁酸异戊酯(Exp2)疏水性的影响是什么？ 2DG的吸收模式(实验3)；碳链长度的影响是什么 和2DG焦点上的末端官能团(Exp.4)； 直链脂肪族化合物6和8的末端官能团 2DG吸收模式上的碳(实验5)；2DG摄取模式 由无味化合物刺激所致(实验6)；以及更改 2DG摄取模式作为刺激浓度变化的函数 化合物(实验7)。

DESCRIPTION: Seven experiments are described in this proposal to study neural activity in the olfactory bulb as the basis of stimulus coding in the olfactory system. Preliminary data indicates that isoamyl acetate and isoamyl butyrate activate the same regions of the olfactory bulb glomerular layer, but that these regions of the olfactory bulb are not activated by ethyl acetate of ethyl butyrate. This finding suggests that isoamyl may be an olfactory "primitive," "odotype," or "epitope." Experiment 1 will determine if isoamyl propionate also activates these same regions and the activation pattern by this odorant will be compared to ethyl propionate. A second part of this experiment will determine if the branched structure or number of hydrocarbons on the "O-side" of the ester bond lead to differential activation of these regions. Subsequent experiments address issues such as, is the same glomerulus activated by isoamyl acetate and isoamyl butyrate (Exp. 2) ; what is the influence of hydrophobicity on patterns of 2DG uptake (Exp.3); what is the effect of carbon chain length and terminal functional group on 2DG foci (Exp.4); what is the effect of the terminal functional group of straight-chain alphatic compounds of 6 and 8 carbons on the pattern of 2DG uptake (Exp. 5); 2DG uptake patterns resulting from stimulation with odorless compounds (Exp. 6); and changes in 2DG uptake patterns as a function of changes in concentration of stimulating compounds (Exp. 7).